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Characterization and Structural Determination of Cold-Adapted Monodehydroascorbate Reductase, MDHAR, from the Antarctic Hairgrass Deschampsia Antarctica

Cited 1 time in wos
Cited 2 time in scopus
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Title
Characterization and Structural Determination of Cold-Adapted Monodehydroascorbate Reductase, MDHAR, from the Antarctic Hairgrass Deschampsia Antarctica
Other Titles
남극 좀새풀 유래 저온 적응 MDHAR 효소의 특성 및 구조 결정
Authors
Park, Ae Kyung
Kim, Il-Sup
Do, Hackwon
Kim, Hyun
Choi, Woong
Jo, Seung-Woo
Shin, Seung Chul
Lee, Jun Hyuck
Yoon, Ho-Sung
Kim, Han-Woo
Subject
Crystallography; Materials Science
Keywords
Antarctic hairgrass; cold adaptation; monodehydroascorbate reductase
Issue Date
2019-10
Citation
Park, Ae Kyung, et al. 2019. "Characterization and Structural Determination of Cold-Adapted Monodehydroascorbate Reductase, MDHAR, from the Antarctic Hairgrass Deschampsia Antarctica". CRYSTALS, 9(10): 537-537.
Abstract
Ascorbic acid (AsA) is an abundant component of plants and acts as a strong and active antioxidant. In order to maintain the antioxidative capacity of AsA, the rapid regeneration of AsA is regulated by dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR). To understand how MDHAR functions under extreme temperature conditions, this study characterized its biochemical properties and determined the crystal structure of MDHAR from the Antarctic hairgrass Deschampsia antarctica (DaMDHAR) at 2.2 A resolution. This allowed for a structural comparison with the mesophilic MDHAR from Oryza sativa L. japonica (OsMDHAR). In the functional analysis, yeast cells expressing DaMDHAR were tolerant to freezing and thawing cycles. It is possible that the expression of DaMDHAR in yeast enhanced the tolerance for ROS-induced abiotic stress
URI
https://repository.kopri.re.kr/handle/201206/10840
DOI
http://dx.doi.org/10.3390/cryst9100537
Appears in Collections  
2019-2019, Application study on the Arctic cold-active enzyme degrading organic carbon compounds (19-19) / Kim, Han-Woo (PN19082)
2019-2019, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (19-19) / Kim, Jin-Hyoung (PE19080)
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