Complete genome sequence of Colwellia hornerae PAMC 20917, a cold-active enzyme-producing bacterium isolated from the Arctic Ocean sediment

Abstract

Psychrophilic bacteria are considered a source of cold-active enzymes that can be used in industrial applications.The Arctic bacterium Colwellia hornerae PAMC 20917 strain has been isolated from the o？shore sediment nearNy-Alesund, Svalbard. The optimal growth temperature of the strain was 10 °C on marine agar. The cell lysateshowed alkaline phosphatase activities. Analysis of the enzymatic properties showed that the alkaline phos-phatase was cold-active and thermolabile. To explore useful cold-active industrial enzymes further, the entiregenome of the PAMC 20917 strain was sequenced. The genome of the strain contained 4,684,314 nucleotides,with 37.87% G+C content. Genome mining analysis revealed that, in the complete genome sequence, threeproteins were annotated as alkaline phosphatases. The genome of PAMC 20917 encodes cold shock proteins andan ice-binding protein that inhibits the growth of ice, allowing the bacterium to adapt to cold environments. Thisgenome information may be useful for understanding mechanisms of adaptation to cold stress.

북극해양침전물로 부터 분리한 Colwellia hornerae PAMC 20917의 유전체 해독 논문: Colwellia의 경우 해양 미생물로서 저온 효소를 많이 가지고 있는 것으로 알려져 있다. 이균주로 부터 저온 알칼라인 포스파타아제를 분비하는 것을 확인하고 유전정보를 확보하기 위해서 유전체 분석을 수행하였다. 3개의 알칼라인 포스파타아제를 유전체에서 확인하였으며, 추가적인 저온 효소로 가치가 있는 아밀라아제, 프로테아제, 글루카나아제 및 풀루란나아제 등을 확인할 수 있었으며, 추가적인 연구로 산업적인 적용에 대해서 고려해 볼 수 있을 것 같다.