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Structural basis of small RNA hydrolysis by oligoribonuclease (CpsORN) from Colwellia psychrerythraea strain 34H

Cited 4 time in wos
Cited 5 time in scopus
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Title
Structural basis of small RNA hydrolysis by oligoribonuclease (CpsORN) from Colwellia psychrerythraea strain 34H
Other Titles
북극 해양미생물 유래 oligoribonuclease (CpsORN) 효소의 구조분석 및 small RNA 분해기작 규명
Authors
Lee, Chang Woo
Park, Sun-Ha
Jeong, Chang-Sook
Cha, Sun-Shin
Park, Hyun
Lee, Jun Hyuck
Subject
Science & Technology - Other Topics
Issue Date
2019-02
Citation
Lee, Chang Woo, et al. 2019. "Structural basis of small RNA hydrolysis by oligoribonuclease (CpsORN) from Colwellia psychrerythraea strain 34H". SCIENTIFIC REPORTS, 9(1): 1-11.
Abstract
Cells regulate their intracellular mRNA levels by using specific ribonucleases. Oligoribonuclease (ORN) is a 3?-5?exoribonuclease for small RNA molecules, important in RNA degradation and re-utilisation. However, there is no structural information on the ligand-binding form of ORNs. In this study, the crystal structures of oligoribonuclease from Colwellia psychrerythraea strain 34H (CpsORN) were determined in four different forms: unliganded-structure, thymidine 5?-monophosphate p-nitrophenyl ester (pNP-TMP)-bound, two separated uridine-bound, and two linked uridine (U-U)-bound forms. The crystal structures show that CpsORN is a tight dimer, with two separated active sites and one divalent metal cation ion in each active site. These structures represent several snapshots of the enzymatic reaction process, which allowed us to predict a possible one-metal-dependent reaction mechanism for CpsORN. Moreover, the biochemical data support our suggested mechanism and identified the key residues responsible for enzymatic catalysis of CpsORN.
URI
https://repository.kopri.re.kr/handle/201206/10880
DOI
http://dx.doi.org/10.1038/s41598-019-39641-0
Appears in Collections  
2019-2019, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (19-19) / Kim, Jin-Hyoung (PE19080)
2019-2019, Development of potential candidates as antibiotics based on polar genetic resources (19-19) / Lee, Jun Hyuck (PE19210)
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