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Skin transcriptome profiling reveals the distinctive molecular effects of temperature changes on Antarctic bullhead notothen

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Title
Skin transcriptome profiling reveals the distinctive molecular effects of temperature changes on Antarctic bullhead notothen
Other Titles
온도 변화에 따른 남극 대구의 발현유전체 변화 분석
Authors
Kim, Bo-Mi
Ahn, Do Hwan
Kang, Seunghyun
Jeong, Jihye
Jo, Euna
Kim, Bum-Keun
Kim, Jin-Hyoung
Park, Hyun
Subject
Biochemistry & Molecular Biology; Toxicology
Keywords
Antarctic bullhead notothen; Notothenia coriiceps; Transcriptome; Temperature; Skin;
Issue Date
2019-04
Citation
Kim, Bo-Mi, et al. 2019. "Skin transcriptome profiling reveals the distinctive molecular effects of temperature changes on Antarctic bullhead notothen". MOLECULAR & CELLULAR TOXICOLOGY, 15(2): 163-172.
Abstract
Backgrounds: Temperature is an important abiotic factor that directly influences the physiology of marine fish. The Antarctic bullhead notothen Notothenia coriiceps inhabits water with temperatures ranging from - 1.9 to 2°C at circumpolar regions. Thus, N. coriiceps is useful as a model animal for understanding the effects of temperature stress. Methods: To assess the transcriptional response of skin tissue to temperature changes, Antarctic bullhead notothen were exposed to two temperature stresses, 4°C and - 2°C, following acclimatization at 2°C. Twenty-four hours after the temperature change, skin transcriptomes were sequenced using the Illumina Hiseq 2000 platform and analyzed using a series of bioinformatics tools. Functional gene annotations through pathway analyses of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed commonly or distinctively modulated transcriptional changes in skin. Results: Both temperature stressors significantly upregulated the actin cytoskeleton regulation pathway and the skin's water barrier function, while the stressors downregulated the metabolism involved in muscle contraction, choline receptor regulation, collagen regulation, and immunity. Cold stress caused significant downregulation of the mRNA expression of genes involved in vasopressin-regulated water reabsorption. Neither the heat- nor cold-stressed skin transcriptomes exhibited significant heat shock protein expression. Conclusion: Our results suggest that, as a first barrier for fish, the skin has complex metabolisms with high transcriptional sensitivity against environmental temperature stress. These results will be useful for understanding the skin-specific molecular mechanisms that Antarctic fish use to adapt to temperature fluctuations. Keywords: Antarctic bullhead notothen, Notothenia coriiceps, Temperature, Skin, Transcriptome
URI
https://repository.kopri.re.kr/handle/201206/10899
DOI
http://dx.doi.org/10.1007/s13273-019-0020-1
Appears in Collections  
2019-2019, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (19-19) / Kim, Jin-Hyoung (PE19080)
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