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Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium

Cited 4 time in wos
Cited 5 time in scopus
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Title
Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium
Other Titles
남극 호냉성 박테리아 유래 신규 저온성 S-Formylglutathione Hydrolase (SfSFGH) 효소의 구조-기능 연구
Authors
Lee, Chang Woo
Yoo, Wanki
Park, Sun-Ha
Lee, Ly Thi Huong Luu
Jeong, Chang?Sook
Ryu, Bum Han
Shin, Seung Chul
Kim, Han-Woo
Park, Hyun
Kim, Kyeong Kyu
Kim, T. Doohun
Lee, Jun Hyuck
Subject
Biotechnology & Applied Microbiology
Keywords
Crystal structure; S-formylglutathione hydrolase; Shewanella frigidimarina; mutagenesis; substrate specificity
Issue Date
2019-08
Citation
Lee, Chang Woo, et al. 2019. "Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium". MICROBIAL CELL FACTORIES, 18(140): 1-13.
Abstract
Background: S-formylglutathione is hydrolyzed to glutathione and formate by an S-formylglutathione hydrolase (SFGH) (3.1.2.12). This thiol esterase belongs to the esterase family and is also known as esterase D. SFGHs contain highly conserved active residues of Ser-Asp-His as a catalytic triad at the active site. Characterization and investigation of SFGH from Antarctic organisms at the molecular level is needed for industrial use through protein engineering. Results: A novel cold-active S-formylglutathione hydrolase (SfSFGH) from Shewanella frigidimarina, composed of 279 amino acids with a molecular mass of ~31.0 kDa, was characterized. Sequence analysis of SfSFGH revealed a conserved pentapeptide of G-X-S-X-G found in various lipolytic enzymes along with a putative catalytic triad of Ser148-Asp224-His257. Activity analysis showed that SfSFGH was active towards short-chain esters, such as p-nitrophenyl acetate, butyrate, hexanoate, and octanoate. The optimum pH for enzymatic activity was slightly alkaline (pH 8.0). To investigate the active site configuration of SfSFGH, we determined the crystal structure of SfSFGH at 2.32 A resolution. Structural analysis shows that a Trp182 residue is located at the active site entrance, allowing it to act as a gatekeeper residue to control substrate binding to SfSFGH. Moreover, SfSFGH displayed more than 50% of its initial activity in the presence of various chemicals, including 30% EtOH, 1% Triton X-100, 1% SDS, and 5 M urea. Conclusions: Mutation of Trp182 to Ala allowed SfSFGH to accommodate a longer chain of substrates. It is thought that the W182A mutation increases the substrate-binding pocket and decreases the steric effect for larger substrates in SfSFGH. Consequently, the W182A mutant has a broader substrate specificity compared to wild-type SfSFGH. Taken together, this study provides useful structure-function data of a SFGH family member and may inform protein engineering strategies for industrial applications of SfSFGH.
URI
https://repository.kopri.re.kr/handle/201206/10939
DOI
http://dx.doi.org/10.1186/s12934-019-1190-1
Appears in Collections  
2019-2019, Application study on the Arctic cold-active enzyme degrading organic carbon compounds (19-19) / Kim, Han-Woo (PN19082)
2019-2019, Development of potential candidates as antibiotics based on polar genetic resources (19-19) / Lee, Jun Hyuck (PE19210)
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