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Genomic and metatranscriptomic analyses of carbon remineralization in an Antarctic polynya

Cited 5 time in wos
Cited 5 time in scopus
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Title
Genomic and metatranscriptomic analyses of carbon remineralization in an Antarctic polynya
Other Titles
남극 폴리냐에서의 탄소 재광물화에 대한 게놈과 메타전사체 해석
Authors
Kim, So-Jeong
Kim, Jong-Geol
Lee, Sang Hoon
Park, Soo-Je
Gwak, Joo-Han
Jung, Man-Young
Chung, Won-Hyung
Yang, Eun Jin
Park, Jisoo
Jung, Jinyoung
Hahn, Yoonsoo
Cho, Jang-Cheon
Madsen, Eugene L.
Rodriguez-Valera, Francisco
Hyun, Jung-Ho
Rhee, Sung-Keun
Subject
Microbiology
Keywords
Carbon remineralization; Genomics; Metatranscriptomics; Polynya
Issue Date
2019-02
Citation
Kim, So-Jeong, et al. 2019. "Genomic and metatranscriptomic analyses of carbon remineralization in an Antarctic polynya". MICROBIOME, 7(29): 1-15.
Abstract
Polynyas in the Southern Ocean are regions of intense primary production, mainly by Phaeocystis antarctica. Carbon fixed by phytoplankton in the water column is transferred to higher trophic levels, and finally, to the deep ocean. However, in the Amundsen Sea, most of this organic carbon does not reach the sediment but is degraded in the water column due to high bacterial heterotrophic activity. We reconstructed 12 key bacterial genomes from different phases of bloom and analyzed the expression of genes involved in organic carbon remineralization. A high correlation of gene expression between the peak and decline phases was observed in an individual genome bin-based pairwise comparison of gene expression. Polaribacter belonging to Bacteroidetes was found to be dominant in the peak phase, and its transcriptional activity was high (48.9% of the total mRNA reads). Two dominant Polaribacter bins had the potential to utilize major polymers in P. antarctica, chrysolaminarin and xylan, with a distinct set of glycosyl hydrolases. In the decline phase, Gammaproteobacteria (Ant4D3, SUP05, and SAR92), with the potential to utilize low molecular weight-dissolved organic matter (LMW-DOM) including compatible solutes, was increased. The versatility of Gammaproteobacteria may contribute to their abundance in organic carbon-rich polynya waters, while the SAR11 clade was found to be predominant in the sea ice-covered oligotrophic ocean. SAR92 clade showed transcriptional activity for utilization of both polysaccharides and LMW-DOM; this may account for their abundance both in the peak and decline phases. Ant4D3 clade was dominant in all phases of the polynya bloom, implicating the crucial roles of this clade in LMWDOM remineralization in the Antarctic polynyas. Genomic reconstruction and in situ gene expression analyses revealed the unique metabolic potential of dominant bacteria of the Antarctic polynya at a finer taxonomic level. The information can be used to predict temporal community succession linked to the availability of substrates derived from the P. antarctica bloom. Global warming has resulted in compositional changes in phytoplankton from P. antarctica to diatoms, and thus, repeated parallel studies in various polynyas are required to predict global warming-related changes in carbon remineralization.
URI
https://repository.kopri.re.kr/handle/201206/10950
DOI
http://dx.doi.org/10.1186/s40168-019-0643-4
Appears in Collections  
2019-2019, Ocean-to-Ice Interactions in Amundsen Sea: Ice shelf melting and its impact on ocean processes (19-19) / Kim, Tae-Wan (PE19060)
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