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Type II Ice-Binding Proteins Isolated from an Arctic Microalga Are Similar to Adhesin-Like Proteins and Increase Freezing Tolerance in Transgenic Plants

Cited 2 time in wos
Cited 2 time in scopus
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Title
Type II Ice-Binding Proteins Isolated from an Arctic Microalga Are Similar to Adhesin-Like Proteins and Increase Freezing Tolerance in Transgenic Plants
Other Titles
북극미세조류로부터 분리된 type II 얼음결합단백질의 특성 및 기능 연구
Authors
Cho, Sung-Mi
Kim, Sanghee
Cho, Hojin
Lee, Hyoungseok
Lee, Jun Hyuck
Lee, Horim
Park, Hyun
Kang, Seunghyun
Choi, Han-Gu
Lee, Jong Eun
Subject
Plant Sciences; Cell Biology
Keywords
Adhesin-like protein; Chloromonas; Ice-binding protein; Freezing tolerance; Ice recrystallization inhibition
Issue Date
2019-12
Citation
Cho, Sung-Mi, et al. 2019. "Type II Ice-Binding Proteins Isolated from an Arctic Microalga Are Similar to Adhesin-Like Proteins and Increase Freezing Tolerance in Transgenic Plants". PLANT AND CELL PHYSIOLOGY, 60(12): 2744-2757.
Abstract
Microalgal ice-binding proteins (IBPs) in the polar region are poorly understood at the genome-wide level, although they are important for cold adaptation. Through the transcriptome study with the Arctic green alga Chloromonas sp. KNF0032, we identified six Chloromonas IBP genes (CmIBPs), homologous with the previously reported IBPs from Antarctic snow alga CCMP681 and Antarctic Chloromonas sp. They were organized with multiple exon/intron structures and low-temperature-responsive cis-elements in their promoters and abundantly expressed at low temperature. The biological functions of three representative CmIBPs (CmIBP1, CmIBP2 and CmIBP3) were tested using in vitro analysis and transgenic plant system. CmIBP1 had the most effective ice recrystallization inhibition (IRI) activities in both in vitro and transgenic plants, and CmIBP2 and CmIBP3 had followed. All transgenic plants grown under nonacclimated condition were freezing tolerant, and especially 35S::CmIBP1 plants were most effective. After cold acclimation, only 35S::CmIBP2 plants showed slightly increased freezing tolerance. Structurally, the CmIBPs were predicted to have β-solenoid forms with parallel β-sheets and repeated TXT motifs. The repeated TXT structure of CmIBPs appears similar to the AidA domain-containing adhesin-like proteins from methanogens. We have shown that the AidA domain has IRI activity as CmIBPs and phylogenetic analysis also supported that the AidA domains are monophyletic with ice-binding domain of CmIBPs, and these results suggest that CmIBPs are a type of modified adhesins.
URI
https://repository.kopri.re.kr/handle/201206/10993
DOI
http://dx.doi.org/10.1093/pcp/pcz162
Appears in Collections  
2019-2019, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (19-19) / Kim, Jin-Hyoung (PE19080)
2019-2019, Modeling responses of terrestrial organisms to environmental changes on King George Island (19-19) / Lee, Hyoungseok (PE19090)
2019-2019, Large-scale production and Clinical evaluation of CPS (Cell-Protecting Substance) from polar microalgae (19-19) / Kim, Sanghee (PE19180)
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