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Effects of a Δ-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties

Cited 2 time in wos
Cited 2 time in scopus
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Title
Effects of a Δ-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties
Other Titles
신규 저온균주 Pseudomonas sp. B14-6에서 유래한 Δ-9- 지방산불포화효소와 싸이클로프로판-지방산합성효소가 세균 막 특성에 끼치는 효과
Authors
Choi, Tae-Rim
Park, Ye-Lim
Song, Hun-Suk
Lee, Sun Mi
Park, Sol Lee
Lee, Hye Soo
Kim, Hyun-Joong
Bhatia, Shashi Kant
Gurav, Ranjit
Lee, Yoo Kyung
Sung, Changmin
Yang, Yung-Hun
Subject
Biotechnology & Applied Microbiology
Keywords
Pseudomonas strain; Phospholipid fatty acid; Genome sequencing; Hydrophobicity; Membrane fluidity
Issue Date
2020-12
Citation
Tae Rim Choi, et al. 2020. "Effects of a Δ-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties". JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 47(1): 1045-1057.
Abstract
Psychrophilic bacteria, living at low and mild temperatures, can contribute significantly to our understanding of microbial responses to temperature, markedly occurring in the bacterial membrane. Here, a newly isolated strain, Pseudomonas sp. B14-6, was found to dynamically change its unsaturated fatty acid and cyclic fatty acid content depending on temperature which was revealed by phospholipid fatty acid (PLFA) analysis. Genome sequencing yielded the sequences of the genes Δ-9-fatty acid desaturase (desA) and cyclopropane-fatty acid-acyl-phospholipid synthase (cfa). Overexpression of desA in Escherichia coli led to an increase in the levels of unsaturated fatty acids, resulting in decreased membrane hydrophobicity and increased fluidity. Cfa proteins from different species were all found to promote bacterial growth, despite their sequence diversity. In conclusion, PLFA analysis and genome sequencing unraveled the temperature-related behavior of Pseudomonas sp. B14-6 and the functions of two membrane-related enzymes. Our results shed new light on temperature-dependent microbial behaviors and might allow to predict the consequences of global warming on microbial communities.
URI
https://repository.kopri.re.kr/handle/201206/11790
DOI
http://dx.doi.org/10.1007/s10295-020-02333-0
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2020 Polar Academic Program (PE20900)
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