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Wide-open conformation of UDP-MurNc-tripeptide ligase revealed by the substrate-free structure of MurE from Acinetobacter baumannii

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Title
Wide-open conformation of UDP-MurNc-tripeptide ligase revealed by the substrate-free structure of MurE from Acinetobacter baumannii
Other Titles
병원균 (Acinetobacter baumannii) 유래 항생물질 타겟 단백질인 MurE 효소의 기질이 결합되어 있지않은 열려진 형태의 구조 규명
Authors
Jung, Kyoung Ho
Kim, Yeon-Gil
Kim, Chang Min
Ha, Hyun Ji
Lee, Chang Sup
Lee, Jun Hyuck
Park, Hyun Ho
Subject
Biochemistry & Molecular Biology; Biophysics; Cell Biology
Keywords
Acinetobacter baumannii; ATP-dependent ligase; cell wall peptidoglycan biosynthesis; crystal structure; MurE
Issue Date
2021-01
Citation
Jung, Kyoung Ho, et al. 2021. "Wide-open conformation of UDP-MurNc-tripeptide ligase revealed by the substrate-free structure of MurE from Acinetobacter baumannii". FEBS LETTERS, 595(2): 275-283.
Abstract
MurE ligase catalyzes the attachment of meso-diaminopimelic acid to the UDP-MurNAc-L -Ala-D -Glu using ATP and producing UDP-MurNAc-L -Ala-D -Glu-meso-A2 pm during bacterial cell wall biosynthesis. Owing to the critical role of this enzyme, MurE is considered an attractive target for antibacterial drugs. Despite extensive studies on MurE ligase, the structural dynamics of its conformational changes are still elusive. In this study, we present the substrate-free structure of MurE from Acinetobacter baumannii, which is an antibiotic-resistant superbacterium that has threatened global public health. The structure revealed that MurE has a wide-open conformation and undergoes wide-open, intermediately closed, and fully closed dynamic conformational transition. Unveiling structural dynamics of MurE will help to understand the working mechanism of this ligase and to design next-generation antibiotics targeting MurE.
URI
https://repository.kopri.re.kr/handle/201206/11815
DOI
http://dx.doi.org/10.1002/1873-3468.14007
Appears in Collections  
2020-2020, Development of potential candidates as antibiotics based on polar genetic resources (20-20) / Lee, Jun Hyuck (PM20030)
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