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The Effect of Translationally Controlled Tumor Protein (TCTP) of the Arctic Copepod Calanus glacialis on Protecting Escherichia coli Cells against Oxidative Stress

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Title
The Effect of Translationally Controlled Tumor Protein (TCTP) of the Arctic Copepod Calanus glacialis on Protecting Escherichia coli Cells against Oxidative Stress
Other Titles
북극 동물플랑크톤 Calanus glacialis TCTP (Translationally Controlled Tumor Protein)가 산화적 스트레스 상태에서 E. coli 세포의 저항성에 미치는 효과
Authors
Park, Yu Kyung
Lee, Chang-Eun
Lee, Hyoungseok
Koh, Hye Yeon
Kim, Sojin
Lee, Sung Gu
Kim, Jung Eun
Yim, Joung Han
Hong, Ju-Mi
Kim, Ryeo-Ok
Han, Se Jong
Kim, Il-Chan
Subject
natural science
Keywords
Antioxidant; arctic copepod; Calanus glacialis; recombinant TCTP; TCTP
Issue Date
2020-10
Citation
Park, Yu Kyung, et al. 2020. "The Effect of Translationally Controlled Tumor Protein (TCTP) of the Arctic Copepod Calanus glacialis on Protecting Escherichia coli Cells against Oxidative Stress". 생명과학회지, 30(11): 931-938.
Abstract
Translationally controlled tumor protein (TCTP) is one of the most abundant proteins in various eukaryotic organisms. TCTPs play important roles in cell physiological processes, being implicated in cancer, cell proliferation, gene regulation, and heat shock response. TCTP is also considered an important factor in the resistance to oxidative stress induced by dithiothreitol or hydrogen peroxide (H2O2). The Arctic calanoid copepods have a variety of antioxidant defense systems to regulate the levels of potentially harmful reactive oxygen species generated by ultraviolet radiation in the Arctic marine ecosystem. However, information on the antioxidant activity of TCTP in the Arctic Calanus glacialis is still scarce. To understand the putative antioxidant function of the Arctic copepod C. glacialis TCTP (Cg-TCTP), its gene was cloned and sequenced. The Cg-TCTP comprised 522 bp and encoded a 174-amino acid putative protein with a calculated molecular weight of ~23 kDa. Recombinant Cg-TCTP (Cg-rTCTP) gene was overexpressed in Escherichia coli (BL21), and Cg-rTCTP-transformed cells were grown in the presence or absence of H2O2. Cg-rTCTP-transformed E. coli showed increased tolerance to high H2O2 concentrations. Therefore, TCTP may be an important antioxidant protein related to C. glacialis tolerance to oxidative stress in the harsh environment of the Arctic Ocean.
URI
https://repository.kopri.re.kr/handle/201206/11844
DOI
http://dx.doi.org/10.5352/JLS.2020.30.11.931
Appears in Collections  
2020-2020, Commercialization of new Biomaterials from polar organisms (20-20) / Yim, Joung Han (PE20010)
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