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Heterologous Gene Expression System Using the Cold-Inducible CnAFP Promoter in Chlamydomonas reinhardtii

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Cited 1 time in scopus
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Title
Heterologous Gene Expression System Using the Cold-Inducible CnAFP Promoter in Chlamydomonas reinhardtii
Other Titles
극지 규조류에서 발굴한 AFP 프로모터를 이용해 저온활성 발현시스템 개발
Authors
Kim, Minjae
Kim, Jongrae
Kim, Sanghee
Jin, EonSeon
Subject
Biotechnology & Applied Microbiology; Microbiology
Keywords
Cold-inducible expression system; antifreeze protein; truncated promoter; Chlamydomonas reinhardtii; Gaussia luciferase; fluorescent protein
Issue Date
2020-11
Citation
Kim, Minjae, et al. 2020. "Heterologous Gene Expression System Using the Cold-Inducible CnAFP Promoter in Chlamydomonas reinhardtii". JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 30(11): 1777-1874.
Abstract
To increase the availability of microalgae as producers of valuable compounds, it is necessary to develop novel systems for gene expression regulation. Among the diverse expression systems available in microalgae, none are designed to induce expression by low temperature. In this study, we explored a cold-inducible system using the antifreeze protein (AFP) promoter from a polar diatom, Chaetoceros neogracile. A vector containing the CnAFP promoter (pCnAFP) was generated to regulate nuclear gene expression, and reporter genes (Gaussia luciferase (GLuc) and mVenus fluorescent protein (mVenus)) were successfully expressed in the model microalga, Chlamydomonas reinhardtii. In particular, under the control of pCnAFP, the expression of these genes was increased at low temperature, unlike pAR1, a promoter that is widely used for gene expression in C. reinhardtii. Promoter truncation assays showed that cold inducibility was still present even when pCnAFP was shortened to 600 bp, indicating the presence of a low-temperature response element between -600 and -477 bp. Our results show the availability of new heterologous gene expression systems with cold-inducible promoters and the possibility to find novel low-temperature response factors in microalgae. Through further improvement, this cold-inducible promoter could be used to develop more efficient expression tools.
URI
https://repository.kopri.re.kr/handle/201206/12047
DOI
http://dx.doi.org/10.4014/jmb.2007.07024
Appears in Collections  
2019-2019, Large-scale production and Clinical evaluation of CPS (Cell-Protecting Substance) from polar microalgae (19-19) / Kim, Sanghee (PE19180)
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