Antarcticibacterium arcticum sp. nov., a bacterium isolated from marine sediment of the Canadian Beaufort Sea

Abstract

A Gram-staining-negative, aerobic, yellow-pigmented, flexirubin-negative, rod-shaped, and non-motile bacterial strain, PAMC 28998T, was isolated from the marine sediment of the Canadian Beaufort Sea. Strain PAMC 28998T grew at 437 C (optimally at 25 C), at pH 7.09.0 (optimally at 7.5) and in the presence of 1.010.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain PAMC 28998T belongs to the genus Antarcticibacterium showing the highest sequence similarity (96.8 %) with A. flavum JB01H24T. Average nucleotide identity and the genome-to-genome distance between PAMC 28998T and the most closely related species A. flavum JB01H24T were 74.1 % and 18.5 %, respectively, indicating that strain PAMC 28998T is clearly distinguished from A. flavum. The genomic DNA G+C content calculated from genome sequences was 39.8 mol%. The major fatty acids (>10 %) were iso-C15:0 (19.5 %), anteiso-C15:0 (18.0 %), iso-C16:0 (11.6 %), summed feature 3 (C16:1 ω6c and/or C16:1 ω7c) (11.4 %). The major polar lipids were phosphatidylethanolamine, aminoglycolipid, two unidentified aminolipids, three unidentified phospholipids, and four unidentified lipids. The major respiratory quinone was MK-6. Based on the phylogenetic, genomic, and phenotypic data presented, strain PAMC 28998T is considered to represent a novel species of the genus Antarcticibacterium, for which the name Antarcticibacterium arcticum sp. nov. is proposed with the strain PAMC 28998T (= KCCM 43316 T = JCM 33514T).