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Ice-binding proteins isolated from Arctic microalgae play a role in freezing tolerance and its regulatory pathway could be associated with AP2 domain containing transcription factor

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Title
Ice-binding proteins isolated from Arctic microalgae play a role in freezing tolerance and its regulatory pathway could be associated with AP2 domain containing transcription factor
Other Titles
북극 미세조류 유래 얼음결합 단백질은 냉해저항성에 주요한 역할을 하고, 이것의 조절 경로는 AP2 전사인자와 연관이 있다.
Authors
Cho, Sung Mi
Kim, Sanghee
Cho, Hojin
Lee, Chang Woo
Lee, Jun Hyuck
Park, Ha Ju
Choi, Han-Gu
Lee, Jungeun
Issue Date
2018
Citation
Cho, Sung Mi, et al. 2018. Ice-binding proteins isolated from Arctic microalgae play a role in freezing tolerance and its regulatory pathway could be associated with AP2 domain containing transcription factor. 2018 International Conferene of the Korean Society of Plant Biologists. Busan, Hotel Nongshim. 2018.11.07~2018.11.09.
Abstract
Polar microalgae have built their own strategies to protect the cells under an environment with repeated freeze-thaw cycles of ice crystals. Microalgal ice-binding proteins (IBPs) are poorly understood at the genome-wide level, although they are essential for cold adaptation. We examined the biological functions of IBPs in the Arctic green alga Chloromonas sp. KNF0032. We identified six IBP genes (called CmIBPs) in a transcriptome study and characterized their structural features. Progressively CmIBP genes were more expressed at low temperature. The CmIBPs were predicted to have β-solenoid forms with parallel β-sheets with repeated TXT motifs. CmIBP1 showed effective ice recrystallization inhibition (IRI) activities both in vitro and in vivo. CmIBP2 and CmIBP3 had moderate or low IRI effects as recombinant proteins, and transgenic plants containing them showed a freezing tolerance phenotype after cold acclimation with increased protein stability. The repeated TXT structure of CmIBPs appears similar to the AidA domain-containing adhesin protein from methanogens. Additionally, it is highlighted that they were organized with multi-exon/intron structures and plant type low-temperature-responsive cis-elements in their promoters. These results suggest that low temperature response pathway in microalgae could be resembled with plants. The regulatory pathway associated with AP2 domain containing transcription factors will be discussed.
URI
https://repository.kopri.re.kr/handle/201206/12278
Conference Name
2018 International Conferene of the Korean Society of Plant Biologists
Conference Place
Busan, Hotel Nongshim
Conference Date
2018.11.07~2018.11.09
Appears in Collections  
2018-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (18-18) / Kim, Jin-Hyoung (PE18080)
2017-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (17-18) / Park, Hyun (PE17080; PE18080)
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