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Functional analysis of AP2 transcription factors from the dominant Antarctic moss Sanionia uncinata

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Title
Functional analysis of AP2 transcription factors from the dominant Antarctic moss Sanionia uncinata
Other Titles
남극 선태류 Sanionia uncinata의 AP2 전사인자 기능 분석
Authors
Byun, Mi Young
Park, Mira
Park, Ha Ju
Kuberski, Anja
Decker, Eva L.
Reski, Ralf
Lee, Chang Woo
Keywords
AP2 transcription factors; Antarctic moss
Issue Date
2018
Citation
Byun, Mi Young, et al. 2018. Functional analysis of AP2 transcription factors from the dominant Antarctic moss Sanionia uncinata. 2018 International conference of the Korean Society of Plant Biologists. Busan. 2018.11.07~2018.11.09.
Abstract
Antarctica is one of the most extreme conditions for plant growth and its vegetation is mainly dominated by bryophytes. The mosses in Antarctica grow mostly in coastal areas and are expected to have developed various unique physiological/molecular mechanisms to survive in extreme environments. Sanionia uncinata (Hypnales; Amblystegiaceae) is a dominant moss species in the maritime Antarctic and considered as a good target species to investigate genes associated with abiotic stress tolerance of mosses. In this study, we aimed to select and characterize function of a key transcription factor which induces the cold tolerance process in S. uncinata at the molecular level. Based on the transcriptome analysis, we could find twenty genes containing AP2 DNA binding domain, showing transcriptional induction in response to cold stress treatment (2℃). To characterize their functions related to cold tolerance, we generated transgenic plants which constitutively express SuAPL genes using the model moss Physcomitrella patens, exhibiting significantly lower cold tolerance than the Antarctic moss. Some of transgenic mosses displayed enhanced freezing tolerance in terms of electrolyte leakage under freezing conditions, compared to wild-type. In addition, their protonemal growth was accelerated below 8℃ indicating enhanced cold tolerance, while the gametophore development was considerably retarded. These results suggest that SuAPL have important functions in cold adaption of Sanionia uncinata to extreme Antarctic environments and they can cause multiple effects in abiotic stress tolerance and in stem cell development of mosses as well.
URI
https://repository.kopri.re.kr/handle/201206/12282
Conference Name
2018 International conference of the Korean Society of Plant Biologists
Conference Place
Busan
Conference Date
2018.11.07~2018.11.09
Appears in Collections  
2018-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (18-18) / Kim, Jin-Hyoung (PE18080)
2017-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (17-18) / Park, Hyun (PE17080; PE18080)
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