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Enrichment of Uncultured Ammonium Oxidizing and Denitrifying Bacteria from Antarctic Soil

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Title
Enrichment of Uncultured Ammonium Oxidizing and Denitrifying Bacteria from Antarctic Soil
Other Titles
남극 토양 유래 암모늄산화세균과 탈질세균의 농화배양
Authors
Cho, Ahnna
Cho, Yong-Joon
Kim, Ok-Sun
Issue Date
2019
Citation
Cho, Ahnna, Cho, Yong-Joon, Kim, Ok-Sun. 2019. Enrichment of Uncultured Ammonium Oxidizing and Denitrifying Bacteria from Antarctic Soil. 2019 International Meeting of the Microbiological Society of Korea. ICCJEJU, Jeju, Korea. 2019.04.17~2019.04.19.
Abstract
Bacteria are contribution a large role in the nitrogen cycle of ecosystems, especially in limited habitat, such as Antarctica. For the cultivation of bacteria involved in the nitrogen cycle of the Antarctica, three soils of King George Island were enriched for nitrogen fixation (NF), ammonium oxidation (AO) and denitrification (DN). Changes in N2O gas, ammonium, nitrite and nitrate concentrations were measured every 14 days. Ammonium oxidation and denitrification were confirmed by measuring changes in nitrogen concentration, and 16S rRNA gene was amplified and analyzed for bacterial communities. Functional gene amoA, which is an ammonium oxidizing gene, and nirK, nirS and nosZ, denitrifying genes, were confirmed. As a result of the bacterial community analysis, it was found that the culture of ammonium oxidizing bacterium were cultivated with Micavibrio, Oxalobacteraceae and Pseudomonadaceae. Oxalobacteraceae, Pseudomonadaceae and Propionibacteriaceae were cultivated in denitrifying bacteria enriched culture. The denitrification pathway and the dissimilatory nitrite reductase pathway were found in the DN_1B and DN_3A denitrifying bacterium cultured in the SEED profile. The ammonia assimilation pathway was more abundant in AO_2C cultured with ammonium oxidizing bacteria. And environmental genetic analysis of the cultures obtained by enrichment will help to understand the ecological function and role of bacteria in the ecosystem. [Supported by the Korea polar Research Institute (Grant PE19090)]
URI
https://repository.kopri.re.kr/handle/201206/12287
Conference Name
2019 International Meeting of the Microbiological Society of Korea
Conference Place
ICCJEJU, Jeju, Korea
Conference Date
2019.04.17~2019.04.19
Appears in Collections  
2019-2019, Modeling responses of terrestrial organisms to environmental changes on King George Island (19-19) / Lee, Hyoungseok (PE19090)
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