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Cryoprotective Effect and Partial Characterization of a Novel Exopolysaccharide (P-ArcPo 20) Produced by Pseudoalteromonas tetraodonis Strain ArcPo 20

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Title
Cryoprotective Effect and Partial Characterization of a Novel Exopolysaccharide (P-ArcPo 20) Produced by Pseudoalteromonas tetraodonis Strain ArcPo 20
Other Titles
극지유래 박테리아 Pseudoalteromonas tetraodonis Strain ArcPo 20 이 생산하는 다당류의 항동결 효과와 특징에 관한 연구
Authors
Kim, Sung Jin
Kang, Pilsung
Kim, Byung-Gee
Park, Ha Ju
Yim, Joung Han
Keywords
Arctic bacterium; Cryoprotective activity; Exopolysaccharide
Issue Date
2018
Citation
Kim, Sung Jin, et al. 2018. Cryoprotective Effect and Partial Characterization of a Novel Exopolysaccharide (P-ArcPo 20) Produced by Pseudoalteromonas tetraodonis Strain ArcPo 20. The 24th international symposium on polarsciences. korea polar research institute. 2018.05.29~2018.05.30.
Abstract
Marine samples obtained from the Chukchi Sea, Arctic Ocean yielded 22 bacterial strains that secrete polysaccharides. Of those, seven strains produced cryoprotective exopolysaccharides (EPS). The strain ArcPo 20, identified as Pseudoalteromonas tetraodonis by 16S rDNA analysis, demonstrated the highest cryoprotective effect and was selected for the further study. The EPS, P-ArcPo 20, was purified by protease treatment and gel filtration chromatography. EPS characterization was confirmed by the gel permeation chromatography, gas chromatography-mass spectrometry, and Fourier transform infrared spectroscopy analysis. The P-ArcPo 20 cryoprotective activity was characterized using an E. coli viability test. In the presence of 0.5% (w/v) EPS, the E. coli cell survival ratio was 91.22 ± 4.21% over five freeze-thaw cycles. These biochemical characteristics suggest that the EPS P-ArcPo 20 may be useful in cryoprotectant development for biotechnological and medical applications.
URI
https://repository.kopri.re.kr/handle/201206/12619
Conference Name
The 24th international symposium on polarsciences
Conference Place
korea polar research institute
Conference Date
2018.05.29~2018.05.30
Appears in Collections  
2016-2018, Cryo-Biotech (16-18) / Yim, Joung Han; Han, Se Jong (PE16190; PE17100; PE18100)
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