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Purification and characterization of a new cold-active cellulolytic enzyme produced by Pseudoalteromonas sp. ArcC09 from the Arctic Beaufort Sea

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Title
Purification and characterization of a new cold-active cellulolytic enzyme produced by Pseudoalteromonas sp. ArcC09 from the Arctic Beaufort Sea
Other Titles
남극 보퍼트해에서 분리한 Pseudoalteromonas sp. ArcC09 에서 생산된 저온 활성 셀룰라아제 효소의 정제와 특성
Authors
Kim, Min Ju
Park, Ha Ju
Kang, Pilsung
Kim, Il Chan
Yim, Joung Han
Han, Se Jong
Subject
Materials Science
Keywords
Arctic bacteriumCellulolytic enzymeCold-activePseudoalteromonasPurification
Issue Date
2022-05
Citation
Kim, Min Ju, et al. 2022. "Purification and characterization of a new cold-active cellulolytic enzyme produced by Pseudoalteromonas sp. ArcC09 from the Arctic Beaufort Sea". BIORESOURCES, 17(2): 3163-3177.
Abstract
A cold-active endoglucanase-producing bacterium was isolated from the Beaufort Sea of the Arctic Ocean and identified as Pseudoalteromonas sp. ArcC09. Cellulolytic activity of ArcC09 reached a maximum of 60 U/mg when cultivated in ZoBell medium for 72 h at 15 °C. This purified endoglucanase, with a molecular mass of 28 kDa, exhibited maximum activity at pH 7.0 and 55 °C. The ArcC09 endoglucanase exhibited 10% and 36% of its maximal activity even at low temperatures of 5 °C and 15 °C, respectively. However, it showed lower thermal stability than a mesophilic cellulase, which is characteristic of a psychrophilic enzyme. The activity was inhibited by CuSO4, and linear alkylbenzene sulfonate (LAS). These findings supplement the understanding of cold-active endoglucanases and may have commercial applications in enzymatic digestion of cellulosic biomass to fermentable sugars.
URI
https://repository.kopri.re.kr/handle/201206/13559
DOI
http://dx.doi.org/10.15376/biores.17.2.3163-3177
Type
Article
Station
Araon
Indexed
SCIE
Appears in Collections  
2021-2021, Commercialization of new Biomaterials from polar organisms (21-21) / Yim, Joung Han (PE21150)
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