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Genomic Survey and Microsatellite Marker Investigation of Patagonian Moray Cod (Muraenolepis orangiensis)

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Genomic Survey and Microsatellite Marker Investigation of Patagonian Moray Cod (Muraenolepis orangiensis)
Other Titles
Patagonian Moray Cod(Muraenolepis orangiensis)의 게놈 연구 및 Microsatellite 마커 조사
Choi, Eunkyung
Lee, Seung Jae
Jo, Euna
Kim, Jinmu
Parker, Steven J.
Kim, Jeong-Hoon
Park, Hyun
AgricultureVeterinary SciencesZoology
Muraenolepis orangiensisPatagonian moray codmicrosatelliteSSRIllumina
Issue Date
Choi, Eunkyung, et al. 2022. "Genomic Survey and Microsatellite Marker Investigation of Patagonian Moray Cod (Muraenolepis orangiensis)". ANIMALS, 12(13): 1-9.
Simple Summary Patagonian moray cod is known to inhabit the cold waters near Antarctica, and it belongs to the Muraenolepis genus. This genus has seven species, and five of them are recently reported. The Muraenolepis genus has similar morphological characters, and this is a limitation of taxonomical classification. In this study, a genome survey and microsatellite marker analysis were conducted to characterize the genome profile for classification. As a result, genomic data such as genome size and microsatellite motifs were obtained. The Muraenolepididae family of fishes, known as eel cods, inhabits continental slopes and shelves in the Southern Hemisphere. This family belongs to the Gadiformes order, which constitutes one of the most important commercial fish resources worldwide, but the classification of the fish species in this order is ambiguous because it is only based on the morphological and habitat characteristics of the fishes. Here, the genome of Patagonian moray cod was sequenced using the Illumina HiSeq platform, and screened for microsatellite motifs. The genome was predicted to be 748.97 Mb, with a heterozygosity rate of 0.768%, via K-mer analysis (K = 25). The genome assembly showed that the total size of scaffolds was 711.92 Mb and the N50 scaffold length was 1522 bp. Additionally, 4,447,517 microsatellite motifs were identified from the genome survey assembly, and the most abundant motif type was found to be AC/GT. In summary, these data may facilitate the identification of molecular markers in Patagonian moray cod, which would be a good basis for further whole-genome sequencing with long read sequencing technology and chromosome conformation capture technology, as well as population genetics.
Appears in Collections  
2021-2022, Ecosystem Structure and Function of Marine Protected Area (MPA) in Antarctica (21-22) / Kim, Jeong-Hoon (PM21060)
2022-2022, 로스해 해양보호구역의 보존조치 이행에 따른 생태계 변화 연구 (22-22) / Kim, Jeong-Hoon (PM22060)
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