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Production and Characterization of a Recombinant Cold-Active Acetyl Xylan Esterase from Psychrophilic Paenibacillus sp. R4 Strain

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Title
Production and Characterization of a Recombinant Cold-Active Acetyl Xylan Esterase from Psychrophilic Paenibacillus sp. R4 Strain
Other Titles
저온성 Paenibacillus sp. R4 균주 유래 재조합 저온활성 acetyl xylan esterase의 생산과 특성 연구
Authors
Dieu Linh NGUYEN
Hwang, Jisub
Kim, Eun Jae
Lee, Jun Hyuck
Han, Se Jong
Keywords
Carbohydrate esterase 7Cold-active acetyl xylan esteraseEscherichia coliIPTG inductionPaenibacillus sp. R4antibiotic modification
Issue Date
2022
Citation
Dieu Linh NGUYEN, et al. 2022. "Production and Characterization of a Recombinant Cold-Active Acetyl Xylan Esterase from Psychrophilic Paenibacillus sp. R4 Strain". APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 58(4): 428-434.
Abstract
Acetyl xylan esterases (AXEs) hydrolyze the specific ester linkages between acetic acid and xylose units, leading to the deacetylation and depolymerization of xylan. PbAcE, the AXE from the soil-psychrophilic Paenibacillus sp. R4 strain, was produced in recombinant E. coli with 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG). The final dry cell weight and purified PbAcE were 17 g/L and 657 mg/L using a 7-L bioreactor and, 6 g/L and 500 mg/L using a 30-L fermenter, respectively. The hydrolysis activity of PbAcE was approximately 27 U/mg when p-nitrophenyl was used as the substrate. PbAcE was most active at pH 8.0 and it maintained more than 80% activity from pH 4.0 to pH 11.0 compared to the maximum value under the given conditions. Furthermore, PbAcE deacetylated cephalothin and chromomycin A3 antibiotics. The current study demonstrates that the production of PbAcE was successful, and the results may encourage further application studies.
URI
https://repository.kopri.re.kr/handle/201206/13721
DOI
http://dx.doi.org/10.1134/S0003683822040123
Type
Article
Station
해당사항없음
Indexed
SCIE
Appears in Collections  
2022-2022, Development of potential antibiotic compounds using polar organism resources (22-22) / Lee, Jun Hyuck (PM22030)
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