Genome analysis of Arctic soft rot bacterial pathogens and comparative analysis with a Korean isolate

Abstract

Soft rot-causing Pseudomonas spp. strains (G4-2, BA90-3, and CAM17-5) isolated from Arctic rhizosphere soils and a freshwater lake sediment, along with the Korean isolate Pectobacterium carotovorum KACC16999, were used for comparative analysis of metabolic traits and pathogenicity. Each genome consisted of a single circular chromosome and contained no plasmids: 6.1 Mb with a G + C content of 59.47% for G4-2; 7.1 Mb and 60.47% for BA90-3; 6.1 Mb and 59.93% for CAM17-5; 5.3 Mb and 51.57% for KACC 16999. Comparative analysis using RAST server revealed that five Arctic isolates (including Arctic soft rot pathogens Pseudomonas strains N3-W and G2-4) possess broader meta- bolic gene repertoires than KACC16999, particularly in amino acid and carbohydrate metabolism categories. These traits suggest their adaptation to Arctic oligotrophic environments.