CAZyme analysis and functional characterization of a new GH18 chitin hydrolase from Gelidibacter salicanalis PAMC21136
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Title
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CAZyme analysis and functional characterization of a new GH18 chitin hydrolase from Gelidibacter salicanalis PAMC21136
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Other Titles
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남극 토양미생물 (Gelidibacter salicanalis PAMC21136) 에서 유래한 새로운 GH18 키틴 가수분해효소의 CAZyme 분석 및 기능적 특성 규명
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Authors
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Lakshan Paudel
Bashu Dev Pardhe
So-Ra Han
Lee, Jun Hyuck
Tae-Jin Oh
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Keywords
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Antarctic bacteria; CAZyme; Chitooligomers; Exo-chitinase; GH18; N-acetyl D-glucosamine
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Issue Date
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2025-03
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Citation
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Lakshan Paudel, et al. 2025. "CAZyme analysis and functional characterization of a new GH18 chitin hydrolase from Gelidibacter salicanalis PAMC21136". Carbohydrate Polymer Technologies and Applications, 10(0): 100773-0.
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Abstract
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Gelidibacter salicanalis strains remain poorly understood in terms of their genomic attributes and are rarely reported for the degradation of polysaccharides within the niche. Gelidibacter salicanalis PAMC21136, an Antarctic isolate, was sequenced and functionally annotated. 251 genes were classified into the CAZyme families, which includes the highest number of GH family, highlighting peculiarity towards various macro biopolymers. Further, based on domain architecture and multiple sequence alignment, gene belonging to the family18 glycoside hydrolase was identified as chitinase. The gene encoding chitinase (MBJ7879808.1) was successfully cloned and expressed in Escherichia coli BL21 cells. MBJ7879808.1 demonstrated activity towards colloidal chitin but there was no detectable activity towards microcrystalline crab shell chitin. Enzyme exhibited an exo-pattern of hydrolysis on both colloidal chitin, and shorter chain GlcNAc oligomers namely [(GlcNAc)3, (GlcNAc)4, (GlcNAc)5, and (GlcNAc)6] producing GlcNAc as a single final product. Biochemical characterization revealed an optimum activity at 25 °C and pH 7.0. Mn²? ions significantly enhanced the activity by 1.7-fold. Turnover number (Kcat) was 8.6 min?1 and catalytic efficiency (Kcat/Km) was 2.0ml/mg/min towards the substrate colloidal chitin. These findings expanded the polysaccharide degradation capability of Gelidibacter salicanalis PAMC21136 and highlight the biotechnological potential of the GH18 enzyme towards production of chitooligomers.
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URI
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https://repository.kopri.re.kr/handle/201206/16586
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DOI
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http://dx.doi.org/10.1016/j.carpta.2025.100773
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Type
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Article
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Station
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King Sejong Station
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Indexed
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국외기타
- Appears in Collections
- 2025-2025, 미생물-식물 유전체와 대사체 기반 생리활성물질 개발 및 식물 회복력 시스템 구축 (25-25) / 이준혁 (PN25170)
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