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Exogenous abscisic acid enhances freeze-thaw stress tolerance in Antarctic moss Sanionia uncinata through coordinated antioxidant defense and osmoprotectant accumulation

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Title
Exogenous abscisic acid enhances freeze-thaw stress tolerance in Antarctic moss Sanionia uncinata through coordinated antioxidant defense and osmoprotectant accumulation
Other Titles
ABA에 의한 남극 이끼 Sanionia uncinata의 동결 스트레스 내성 증진
Authors
Seo, Suyeon
Ahn, Yong-Yoon
Kim, Kitae
Lee, Jun Hyuck
Min, Kyungwon
Lee, Hyoungseok
Keywords
Abscisic acidAntioxidant enzymesFreeze-thaw stress toleranceSanionia uncinataOsmoprotectants
Issue Date
2025-12
Citation
Seo, Suyeon, et al. 2025. "Exogenous abscisic acid enhances freeze-thaw stress tolerance in Antarctic moss Sanionia uncinata through coordinated antioxidant defense and osmoprotectant accumulation". CRYOBIOLOGY, 121(0): 0-0.
Abstract
Abscisic acid (ABA) is known to improve plant freeze-thaw stress tolerance (FTST), but its role in Antarctic mosses remains largely unexplored. Accordingly, this study investigated the effects of exogenous ABA application on FTST of gametophore Sanionia uncinata, one of the most widespread moss species in maritime Antarctica. Samples were treated with 10 mu M ABA for 72 h under controlled culture conditions, then subjected to freezing stress at -13 and -16 degrees C. Various physiological parameters were measured to assess ABA-induced FTST, including ion-leakage, photosynthetic efficiency (Fv/Fm), malondialdehyde (MDA) content, compatible solutes/osmolytes (proline and total sugars), and antioxidant enzyme activities, e.g., superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). Gametophores treated with ABA efficiently assimilated ABA, evident by similar to 20-fold higher than controls. ABA application did not impede gametophore growth but slightly increased dry weight/fresh weight ratio (5 % increase). ABA significantly improved freezing tolerance, reducing freeze-induced membrane injury by similar to 20 % at both stress temperatures, alleviating oxidative stress with 25-34 % lower MDA accumulation, and enhancing PSII maximum quantum yield (Fv/Fm) by 6-7 % following freeze-thaw stress. Improved FTST was paralleled by enhanced activities of antioxidant enzymes, CAT and APX increasing by similar to 11 % and 56 %, respectively, while SOD remained unchanged. ABA treatment also promoted osmoprotectant accumulation, with proline content increasing by 33.5 % and total soluble sugars nearly doubling (99 % increase). These findings provide the first quantitative evidence that exogenous ABA markedly improves FTST in an Antarctic moss through coordinated antioxidant defense and osmoprotectant accumulation, highlighting ABA as a critical regulator of stress resilience in S. uncinata.
URI
https://repository.kopri.re.kr/handle/201206/16616
DOI
http://dx.doi.org/10.1016/j.cryobiol.2025.105325
Type
Article
Station
King Sejong Station
Indexed
SCIE
Appears in Collections  
2025-2025, 미생물-식물 유전체와 대사체 기반 생리활성물질 개발 및 식물 회복력 시스템 구축 (25-25) / 이준혁 (PN25170)
2025-2025, 환경변화에 따른 남극 육상생물의 생리생태 반응 규명 (25-25) / 이형석 (PE25130)
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