Genomic and phenotypic characteristics of a thermophilic Bacillus sp. 9F isolated from deep-sea hydrothermal vent plume, southern ocean
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- Genomic and phenotypic characteristics of a thermophilic Bacillus sp. 9F isolated from deep-sea hydrothermal vent plume, southern ocean
- Lee, Inae
Hwang, Chung Yeon
Yoon, Soo Jung
Lee, Yung Mi
Hong, Soon Gyu
Park, Sung Hyun
Lee, Hong Kum
- Genome; Bacteria; Southern Ocean; Thermophile; Vent
- Issue Date
- Lee, Inae, et al. 2015. Genomic and phenotypic characteristics of a thermophilic Bacillus sp. 9F isolated from deep-sea hydrothermal vent plume, southern ocean. 6th International Conference on Polar and Alpine Microbiology. University of South Bohemia. 2015.09.06-10.
- The strain 9F was isolated from deep-sea hydrothermal vent plume in Australian-Antarctic Ridge in the Southern
Ocean. Analysis of the 16S rRNA gene sequence of strain 9F showed an affiliation to the type strain of Bacillus
lichneformis with a high similarity of 99.9%. In the present study, we compared genomic and phenotypic features
of strain 9F with those of the type strain of B. lichneformis (KCTC 1918T) to find differences between the strains of
different origins; strain 9F is the first strain from deep-sea hydrothermal vent environment, while other strains B.
licheniformis have been isolated from diverse terrestrial environments including the type strain.
METHODS AND MATERIALS
A genome sequence of strain 9F was determined using an Illumina MiSeq, whereas a complete genome sequence
of the type strain of B. licheniformis was retrieved from the GenBank under the accession AE017333 (4,222,645
bp with the G+C content of 46.2 mol%). Temperature and salinity ranges for growth were determined for both
strains. Enzyme activities, carbon assimilation, acid production from carbohydrates were assayed using the API
ZYM, 20NE and 50CH kits.
A draft genome of strain 9F revealed 29 contigs in 4,376,753 bp with the G+C content of 45.6 mol%. Genomic
relatedness analyses based on average nucleotide identity and the genome-to-genome distance showed that
strain 9F and B. licheniformis KCTC 1918T belonged to single species. A lower limit of temperature for growth was
10°C and 15°C for strain 9F and B. licheniformis KCTC 1918T, respectively, while an upper limit of temperature was
60°C for both strains. Salinity tolerant tests showed an identical result for both strains (0-7.5% NaCl, w/v). No
difference between both strains was found in the enzyme profiles with the API ZYM and 20 NE assays. However,
an obvious difference in acid production (14 out of 49 carbohydrates in the API 50CH assay) and the assimilation
of arabinose were observed between them.
- Conference Name
- 6th International Conference on Polar and Alpine Microbiology
- Conference Place
- University of South Bohemia
- Conference Date
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