Marinobacterium profundum sp. nov., a marine bacterium from deep-sea sediment

Abstract

A Gram-stain-negative, rod-shaped and motile strain, designated PAMC 27536^T, was isolated from deep-sea sediment in the East Sea, Korea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus <em>Marinobacterium</em>. Phylogenetic analyses revealed that strain PAMC 27536^T was most closely related to the type strain of <em>Marinobacterium rhizophilum</em> CL-YJ9^T with a 16S rRNA gene sequence similarity of 98.5 % and to other members of the genus Marinobacterium (94.0-91.7 %). Genomic relatedness analyses between strain PAMC 27536^T and <em>M. rhizophilum</em> KCCM 42386T resulted in the average nucleotide identity of 85.6% and the estimated DNA-DNA hybridization of 24.6 % by the genome-to-genome distance calculator, indicating that they represent genomically distinct species. Cells of strain PAMC 27536^T grew optimally at 25-30 ℃ and pH 7.0-7.5 in the presence of 3 % (w/v) sea salts. The major cellular fatty acids were C16:1 ω6c and/or C16:1 ω7c (42.7 %), C18:1 ω6c and/or C18:1 ω7c (20.9 %) and C16:0 (19.1 %). The major isoprenoid quinone was Q-8. The genomic DNA G+C content was 56.1-57.2 mol%. Based on the phylogenetic, chemotaxonomic, genomic and phenotypic data presented, we propose the name<em> Marinobacterium profundum</em> sp. nov. with the type strain PAMC 27536^T (= KCCM 43095^T = JCM 30410^T).