Marine RNA virus comminities of oceanic seawaters in the vicinity of the antarctic peninsula
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- Marine RNA virus comminities of oceanic seawaters in the vicinity of the antarctic peninsula
- Hwang, Chung Yeon
Mueller, Jaclyn A.
Steward, Grieg F.
- RNA; Virus; Metagenome; Antarctic; Seawater
- Issue Date
- Hwang, Chung Yeon, Mueller, Jaclyn A., Steward, Grieg F.. 2015. Marine RNA virus comminities of oceanic seawaters in the vicinity of the antarctic peninsula. 6th International Conference on Polar and Alpine Microbiology. University of South Bohemia. 2015.09.06-10.
- Viruses play an important role in the biogeochemical cycle and the gene flux in marine plankton ecosystems.
Multiple lines of evidence that RNA viruses comprise a significant fraction of virioplakton are emerging. However,
no data on oceanic RNA viromes in the Antarctic seas are available. During the IBRV Araon cruise in 2013, each 50
L of surface seawaters at 4 offshore stations was collected and filtered to concentrate viruses by the FeCl3
flocculation method. Marine viruses were recovered and purified by the CsCl gradient ultracentrifugation. Nucleic
acids of viral fractions were extracted and subsequently DNAs were removed by adding DNase. Metagenome
libraries were generated from RNAs using the random priming-mediated sequence-independent single-primer
amplification. Size ranges of 300-600 bp in the libraries were selected and sequenced by 454 pyrosequencing with
the GS-FLX Titanium chemistry, yielding 333 Mb with 764,764 reads as raw data in total. In the presentation,
results and its implications will be discussed.
- Conference Name
- 6th International Conference on Polar and Alpine Microbiology
- Conference Place
- University of South Bohemia
- Conference Date
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