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Isolation of Protease-Producing Arctic Marine Bacteria

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Isolation of Protease-Producing Arctic Marine Bacteria
Other Titles
단백질분해효소를 생산하는 북극 해양 세균의 분리
Sung, Ki Cheol
Lee, Yoo Kyung
Yim, Joung Han
Park, Kyu Jin
Lee, Hong Kum
Chung, Hosung
Arctic; Marine bacteria; Protease; Exiguobacterium; Pseudoalteromonas; Pseudomonas
Issue Date
Sung, Ki Cheol, et al. 2005. "Isolation of Protease-Producing Arctic Marine Bacteria". Korea Institute of Ocean Science & Technology, 27: 215-219.
We isolated and identified three protease-producing bacteria that had inhabited the region around the Korean Arctic Research Station Dasan located at Ny-Alesund, Svalbard, Norway (79oN, 12oE). Biofilms were collected from the surface of a floating pier and from dead brown algae in a tide pool near the seashore. The biofilm samples were transported to the Korea Polar Research Institute (KOPRI) under frozen conditions, diluted in sterilized seawater, and cultured on Zobell agar plates with 1% skim milk at 10oC. Three clear zone forming colonies were selected as protease-producing bacteria. Phylogenetic analysis based on 16S rDNA sequences showed that these three stains shared high sequence similarities with Pseudoalteromonas elyakovii, Exiguobacterium oxidotolerum and Pseudomonas jessenii, respectively. We expect these Arctic bacteria may be used to develop new varieties of protease that are active at low temperatures.
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