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Isolation and spectral characterization of cadmium binding metallothionein

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Cited 2 time in scopus
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Isolation and spectral characterization of cadmium binding metallothionein
Other Titles
남극큰띠조개의 중금속 결합 단백질 분리 및 특성 연구
Park, Hyun
Ji, Jung Youn
Ahn, In-Young
Choi, Heeseon J.
Environmental Sciences & Ecology; Physical Geography
Antarctic; Cd-thiolate complex; Laternula; MTs; bivalves
Issue Date
Park, Hyun, et al. 2008. "Isolation and spectral characterization of cadmium binding metallothionein". ANTARCTIC SCIENCE, 20(1): 33-37.
A cadmium (Cd)-binding protein was isolated and characterized from the Antarctic clam Laternula elliptica after experimental exposure to a high concentration of Cd. Cd-binding metallothioneins (MTs) in the cytosol were purified using a procedure based on gel permeation and ion-exchange chromatography. The purified MTs were recognized by MT antibodies in a Western blotting assay. MALDI-TOF MS analyses showed that the molecular mass of the purified MTs was 7.27 kDa, which is typical of MTs found in marine invertebrates. The Cd binding to MT, reflected by the redistribution of Cd ions, was monitored by spectrophotometry. The absorption spectra profiles indicated the presence of Cd-MT complexes, and a 4 nm red shift of the unresolved lowest energy-absorption band occurred when five equivalents of Cd (II) were incorporated.
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