Estimation of antioxidant, antimicrobial activityand brine shrimp toxicity of plants collected fromOymyakon region of the Republic of Sakha(Yakutia), Russia

Abstract

A total of 114 plant species, including 80 species of higher plants, 19 species of lichens and 15 species of mosses, were collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia (63°20′N, 141°42′E？63°15′ N, 142°27′E). Antimicrobial, DPPH free radical scavenging and brine shrimp (Artemia salina) toxicity of all crude extract were evaluated. The obtained result was analyzed and compared with commercial standards. A total of 28 species of higher plants showed very strong antioxidant activity (DPPH IC50, 0.45-5.0 μg/mL), 13 species showed strong activity (DPPH IC50, 5-10 μg/mL), 22 species showed moderate antioxidant activity (DPPH IC50,10-20 μg/mL) and 17 species showed weak antioxidant activity (DPPH IC50 more than 20 μg/mL). Similarly, 3 species of lichen showed strong antioxidant activity, one species showed moderate and 15 species showed weak DPPH reducing activity. In addition, 4 species of mosses showed moderate antioxidant activity and 11 species showed weak antioxidant activity. Similarly, extracts of 51 species of higher plants showed antimicrobial (AM) activity against Staphylococcus aureus and 2 species showed AM activity against Candida albicans. Similarly, 11 species of lichen showed AM activity against S. aureus and 3 species showed AM activity against Escherichia coli. One species of moss showed AM activity against S. aureus. And finally, one species of higher plant Rheum compactum and one species of lichen Flavocetraria cucullata showed the toxicity against Brine shrimp larvae in 100 μg/mL of concentration.