Dasania marina gen. nov., sp nov., of the order Pseudomonadales, isolated from arctic marine sediment

Abstract

An obligately aerobic bacterium, strain KOPRI 20902(T), was isolated from a marine sediment in Ny-Alesund, Spitsbergen Islands, Norway. Cells were irregular rods and motile with polar monotrichous flagellum. The optimum growth temperature was 17-22 degrees C. Cells grew best in pH 7.0-10.0 and 3-4% sea salts (corresponding to 2.3-3.1% NaCl). The novel strain required Ca2+ or Mg2+ in addition to NaCl for growth. Sequence analysis of 16S rRNA gene revealed that the Arctic isolate is distantly related with established species (< 92.4% sequence similarity) and formed a monophyletic group with Cellvibrio, which formed a distinct phylogenetic lineage in the order Pseudomonadales. Predominant cellular fatty acids [C-16:1 omega 7c/15:0 iso 20H (45.3%), C-16:0 (18.4%), ECL 11.799 (11.2%), C-10:0 30H (10.4%)];DNA G+C content (37.0 mol%);nitrate reduction to nitrogen;absence of aesculin hydrolysis, N-acetyl-beta-glucosaminidase and esterase;no assimilation of arabinose, galactose, glucose, lactose, maltose, and trehalose differentiated the strain from the genus Cellvibrio. Based on the phylogenetic and phenotypic characteristics, Dasania marina gen. nov., sp. nov. is proposed in the order Pseudomonadales. Strain KOPRI 20902(T) (=KCTC 12566(T)=JCM 13441(T)) is the type strain of Dasania marina.