Morphology and Molecular Data forAntarctic Cryophilic Microalga, Porosira pseudodenticulata

Abstract

We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections ofpolar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 bymorphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify thestrain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity withGenBank X85398. This result was supported by morphological features like solitary labiate process, externalforamina and internal cribra by optical and scanning electron microscope. Morphological identification andmolecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.