Involvement of a laccase-like multicopper oxidase in humic substances degradation by diverse polar soil bacteria
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- Involvement of a laccase-like multicopper oxidase in humic substances degradation by diverse polar soil bacteria
- Other Titles
- 극지역 토양성 세균 유래 laccase-like multicopper oxidase 효소의 부식질 분해 관련 연구
- Park, Ha Ju
Lee, Jun Hyuck
- Biodegradation; Bipolar; Cold-adapted; Humic acids; Laccase; Soil bacteria
- Issue Date
- Park, Ha Ju, et al. 2016. Involvement of a laccase-like multicopper oxidase in humic substances degradation by diverse polar soil bacteria. ASM microbe 2016. 미국 보스턴. 2016.06.16~2016.06.20.
- Soil humic substances (HS) are widely distributed in cold environments and comprise a significant fraction of soil organic carbon. Bacterial strains (n=281) were isolated at 15°C using a minimal medium containing humic acids (HA), a principal component of HS, from various polar soil samples: 217 strains from the Antarctic and 64 strains from Arctic soil. The 73 potential HA-degrading bacteria were affiliated with phyla Proteobacteria (73.9%), Actinobacteria (20.5%), and Bacteroidetes (5.5%). Most of them degraded HA at 10°C to 25°C, but not at temperatures over 30°C, indicating their cold-adapted degradative abilities. Thirty unique laccase-like multicopper oxidase (LMCO) gene fragments were PCR-amplified from 71% of the isolated HA-degrading bacteria, all of which included the conserved copper binding regions (CBR) I and II, both essential for laccase activity. The bacterial LMCO sequences differed from known fungal laccases
for example, a cysteine residue between CBR I and CBR II of fungal laccases was not detected in the bacteria LMCOs. In addition, computer-aided molecular modeling showed these LMCOs contain a highly conserved copper-dependent active site formed by three histidine residues between CBR I and CBR II. Phylogenetic- and modeling-based methods con？rmed the wide occurrence of LMCO genes in HA-degrading polar soil bacteria and linked their putative gene functions with initial oxidative reaction for HA degradation.
- Conference Name
- ASM microbe 2016
- Conference Place
- 미국 보스턴
- Conference Date
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