KOPRI Repository

A new approach for discovering cold-active enzymes in a cell mixture of pure-cultured bacteria

Cited 3 time in wos
Cited 5 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorKim, Dockyu-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorKim, Il-Chan-
dc.contributor.authorPark, Ha Ju-
dc.date.accessioned2018-03-29T05:58:59Z-
dc.date.available2018-03-29T05:58:59Z-
dc.date.issued2014-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7248-
dc.description.abstractTo overcome the intrinsic problems of conventional approaches, such as the unavailability of source microorganisms in metagenomic libraries and the production of inactive aggregates, a new method was tested for discovering new enzymes (e.g., cold-active chitinase). A metagenome-like library was constructed using genomes extracted from a cell mixture of purely cultured chitinolytic bacteria, followed by activity-based screening for E. coli clones that exhibit chitinase activity on selective medium. Within one positive chitinolytic clone, one chitinase gene (chi22718_III) was detected and assigned to the Arctic marine bacterium Pseudoalteromonas issachenkonii PAMC 22718 by colony-PCR with chi22718_III-specific primers. When expressed in E. coli, recombinant R-Chi22718_III lost 85% of its enzyme activity when pre-incubated at 40°C for 1 h, whereas its mesophilic counterpart R-ChiK only lost 10% of its activity under the same conditions, indicating that R-Chi22718_III is thermolabile, a characteristic of cold-active enzymes.-
dc.languageEnglish-
dc.publisherSpringer-
dc.titleA new approach for discovering cold-active enzymes in a cell mixture of pure-cultured bacteria-
dc.title.alternative순수배양한 세균의 세포혼합액에서 저온활성 효소를 발굴하기 위한 새로운 탐색 방법-
dc.typeArticle-
dc.identifier.bibliographicCitationKim, Dockyu, et al. 2014. "A new approach for discovering cold-active enzymes in a cell mixture of pure-cultured bacteria". <em>BIOTECHNOLOGY LETTERS</em>, 36(3): 567-573.-
dc.citation.titleBIOTECHNOLOGY LETTERS-
dc.citation.volume36-
dc.citation.number3-
dc.identifier.doi10.1007/s10529-013-1384-2-
dc.citation.startPage567-
dc.citation.endPage573-
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2012:58.75-
dc.subject.keywordActivity-based enzyme screen-
dc.subject.keywordChitinase-
dc.subject.keywordFunctional expression-
dc.subject.keywordMetagenomic library-
dc.subject.keywordPseudoalteromonas issachenkonii-
dc.identifier.localId2013-0356-
dc.identifier.scopusid2-s2.0-84897042861-
dc.identifier.wosid000333349300018-
Appears in Collections  
2011-2016, Exploration of Future Resources in The Polar Oceans and Study on Their Utilization (K-POD) (11-16) / Yim; Joung Han
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse