Marinobacterium profundum sp. nov., a marine bacterium from deep-sea sediment

Abstract

A Gram-stain-negative, rod-shaped and motile strain, designated PAMC 27536T, was isolated from deep-sea sediment in the East Sea, Korea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Marinobacterium. Phylogenetic analyses revealed that strain PAMC 27536T was most closely related to the type strain of Marinobacterium rhizophilum CL-YJ9T with a 16S rRNA gene sequence similarity of 98.5 % and to other members of the genus Marinobacterium (94.0？91.7 %). Genomic relatedness analyses between strain PAMC 27536T and M. rhizophilum KCCM 42386T resulted in the average nucleotide identity of 85.6% and the estimated DNA-DNA hybridization of 24.6 % by the genome-to-genome distance calculator, indicating that they represent genomically distinct species. Cells of strain PAMC 27536T grew optimally at 25？30 oC and pH 7.0？7.5 in the presence of 3 % (w/v) sea salts. The major cellular fatty acids were C16:1 ω6c and/or C16:1 ω7c (42.7 %), C18:1 ω6c and/or C18:1 ω7c (20.9 %) and C16:0 (19.1 %). The major isoprenoid quinone was Q-8. The genomic DNA G+C content was 56.1？57.2 mol%. Based on the phylogenetic, chemotaxonomic, genomic and phenotypic data presented, we propose the name Marinobacterium profundum sp. nov. with the type strain PAMC 27536T (= KCCM 43095T = JCM 30410T).