Pseudoruegeria aestuarii sp. nov., of the family Rhodobacteraceae, isolated from a tidal flat

Abstract

Gram staining negative, non-motile, aerobic, and rod-shaped bacterium, designated strain MME-001T, was isolated from the tidal flat of Muui-do in the Republic of Korea.Phylogenetic trees based on the 16S rRNA gene sequence showed that strain MME-001T belonged to the genus Pseudoruegeria in the family Rhodobacteraceae and that it shared the highest 16S rRNA gene sequence similarity with Pseudoruegeria sabulilitoris GJMS-35T (98.0% similarity of the 16S rRNA gene). Growth of this isolate occurred in the presence of 1.0？7.0% (w/v) NaCl at 15？40 ºC and pH 7.0？9.0, with optimal growth in the presence of 2.0？3.0% (w/v) NaCl at 25？30 ºC and pH 7.0. Ubiquinone-10 was the major respiratory quinone. Major polar lipids were PC, PE, PG,and an unidentified lipid. The major cellular fatty acids were C18:1 ω7c and C16:0. The genomic DNA G+C content was 62 mol%. The relatedness of DNA-DNA hybridization of between strain MME-001T and P. sabulilitoris KCTC 42111T, "P. limi" KCTC 32460T, P. lutimaris KCTC 22690T, P. aquimaris KCTC 12737T, and P. haliotis KACC 17214T was 36 ± 5, 57 ± 7, 34 ± 4, 18 ± 5, and 21 ± 3%, respectively. Based on the phenotypic and phylogenetic taxonomical properties, this strain is a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria aestuarii sp. nov. is proposed. The type strain of Pseudoruegeria aestuarii is MME-001T (=KCCM 43133T=JCM 30751T).