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Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027

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Title
Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027
Other Titles
Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027
Authors
이재형
Lee, Yoo Kyung
Lee, Hong Kum
Keywords
BacteriaGeneMarineVibriochitinase
Issue Date
2005
Citation
이재형, Lee, Yoo Kyung, Lee, Hong Kum. 2005. Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027. 국제해양생명공학회. 국제해양생명공학회. 2005.06.11~.
Abstract
The chiK gene encoding chitinase ChiK was isolated from a marine bacterium, Vibrio sp. 98CJ11027. We amplified a partial sequence of the chiK using chitinase-specific primer sets. Using inverse PCR cloning method with two specific sets of PCR primers rather than single set, we identified the 4 kb DNA fragment containing chiK gene. From the fragment, we amplified the full chiK gene and cloned into pUC18 vector designated as pChiK. The recombinant E. coli clones harboring pChiK produced a clear zone around the colony in the colloidal chitin plates. Open reading frame of the chiK is 2553 bp long encoding a ChiK precursor protein of 851 amino acids, which consist of N-terminal signal peptide of 21 amino acids and a mature ChiK protein with 830 amino acids. The deduced amino acid sequences of chiK shows 89% identity with that of Vibrio carchariae chitinase. The conserved module of Vibrio sp. 98CJ11027 ChiK indicates that the ChiK belongs to the family 18 of glycoside hydrolases.
URI
https://repository.kopri.re.kr/handle/201206/7566
Conference Name
국제해양생명공학회
Conference Place
국제해양생명공학회
Conference Date
2005.06.11~
Type
Proceeding
Indexed
Pro(초록)국외
Appears in Collections  
2004-2005, 극지유용생물자원활용기술개발사업 (04-05) / Kang, Sung-Ho (PN04003)
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