Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027
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Title
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Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027
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Other Titles
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Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027
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Authors
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이재형
Lee, Yoo Kyung
Lee, Hong Kum
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Keywords
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Bacteria; Gene; Marine; Vibrio; chitinase
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Issue Date
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2005
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Citation
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이재형, Lee, Yoo Kyung, Lee, Hong Kum. 2005. Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027. 국제해양생명공학회. 국제해양생명공학회. 2005.06.11~.
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Abstract
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The chiK gene encoding chitinase ChiK was isolated from a marine bacterium, Vibrio sp. 98CJ11027. We amplified a partial sequence of the chiK using chitinase-specific primer sets. Using inverse PCR cloning method with two specific sets of PCR primers rather than single set, we identified the 4 kb DNA fragment containing chiK gene. From the fragment, we amplified the full chiK gene and cloned into pUC18 vector designated as pChiK. The recombinant E. coli clones harboring pChiK produced a clear zone around the colony in the colloidal chitin plates. Open reading frame of the chiK is 2553 bp long encoding a ChiK precursor protein of 851 amino acids, which consist of N-terminal signal peptide of 21 amino acids and a mature ChiK protein with 830 amino acids. The deduced amino acid sequences of chiK shows 89% identity with that of Vibrio carchariae chitinase. The conserved module of Vibrio sp. 98CJ11027 ChiK indicates that the ChiK belongs to the family 18 of glycoside hydrolases.
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URI
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https://repository.kopri.re.kr/handle/201206/7566
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Conference Name
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국제해양생명공학회
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Conference Place
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국제해양생명공학회
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Conference Date
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2005.06.11~
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Type
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Proceeding
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Indexed
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Pro(초록)국외
- Appears in Collections
- 2004-2005, 극지유용생물자원활용기술개발사업 (04-05) / Kang, Sung-Ho (PN04003)
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