In vitro propagation of Arctic mouse-ear chickweed in high temperature

Abstract

An effective in vitro preservation and propagation conditions of higher polar plant Arctic Mouse-ear Chickweed (Cerastium arcticum) were investigated in higher temperature. With slightly modified Murashige and Skoog (MS) media, Cerastium arcticum has been able to propagate at higher temperature than its ecological growth temperature. The optimal flowering conditions in growth chamber system were 15℃/10℃ (day/night), 3000±100 Lux, and 21/3h (day/night) of temperature, light, and photoperiod, respectively. For in vitro preservation, suspension culture with modified MS media showed the highest growth rate in the temperature of 23？

1℃. The biomass of suspension cultures Cerastium arcticum were doubled by every 7days after sub-cultured.