Phylogenetic Analysis of Arctic and Antarctic Marine Bacteria

Abstract

The bacteria were isolated from various marine environments, and identified using phylogenetic analysis on the basis of 16S rRNA gene sequences. Sediments, macro algae, marine animal and sea water samples were collected from around the Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway (79°N, 12°E) and Korean Antarctic Research Station King Sejong on King George Island, Antarctica (62°13′ S, 58°47′ W). The collected samples were transported to KOPRI under frozen conditions, diluted in sterilized seawater, and cultured on zobell agar plates at 10℃ and 25℃. Total 386 bacteria isolates were cultured and preserved in glycerol media (15%, v/v) at –80℃. Phylogenetic analysis of the bacterial isolates indicated that they belonged to alpha-Proteobacteria, beta-Proteobacteria, gamma-Proteobacteria, CFB group, and High GC Gram-positive bacteria. We isolated candidates for new species; the closest bacteria with validly published names were Cellulophaga baltica, Colwellia piezophila, Devosia neptuniae, Formosa algae, Janibacter marinus, Jannaschia helgolandensis, Pseudomonas fluorescens, Polaribacter irgensii, Plantibacter flavus, Rheinheimera baltica, Roseobacter gallaeciensis, Pibocella ponta, Sanguibacter keddieii, Subtercola boreus, Thiobacillus prosperus, and Winogradskyella eximia.