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Isolation and characterization of Antarctic bacteria with protease activities

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Isolation and characterization of Antarctic bacteria with protease activities
Other Titles
단백질분해효소 활성을 나타내는 남극 토양 미생물의 분리 및 특성 연구
Yoo, Miyoun
Kim, Eungbin
Sohn, Min Ji
Ahn, Eunsol
Kim, Dockyu
Oh, Yu Jin
Antarctic; Protease; bacteria; screening
Issue Date
Yoo, Miyoun, et al. 2011. Isolation and characterization of Antarctic bacteria with protease activities. 한국미생물학회연합회. 한국미생물학회연합회. 2011.10.14~.
Proteases, being one of the largest groups of industrial enzymes, have been used in a wide range of applications including protein stain removal, fuel, food preparation, biofilm removal, leather preparation, and so on. Microorganisms represent an excellent source of protease enzymes owing to their broad biochemical diversity and their susceptibility to genetic manipulation. As an effort to unearth new microbial resources for proteases, we have screened Antarctic soils for the presence of proteolytic bacteria using casein and keratin as substrates, respectively. A total of 169 bacterial genera were identified including Pseudomonas (109), Bacillus (9), Thermoleophilum (11), Micro- coccus (6), Chryseobacterium (2), Arthrobacter (3), Stenotrophomonas (3), Sporocarcina (2), Paenibacillus (1), Planococcus (1), and unclassified Bacteria (22) after screening 100 different soil samples. Strains showing keratinase activity were further screened for caseinase enzyme activity. 28 strains from 53 strains showing keratinase enzyme activity also showed caseinase enzyme activity.
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