KOPRI Repository

Anthranilate degradation by an eurypsychrophilic Pseudomonas sp. form Alps

Cited 0 time in wos
Cited 0 time in scopus
Metadata Downloads
Anthranilate degradation by an eurypsychrophilic Pseudomonas sp. form Alps
Other Titles
알프스 eurypsychrophilic Pseudomonas sp. KOPRI 25931에 의한 anthranilate 분해 경로 연구
Kim, Dockyu
Park, Ha Ju
Kim, Eungbin
Hong, Soon Gyu
Yoo, Miyoun
Pseudomonas; alpine; anthranilate; eurypsychrophilic
Issue Date
Kim, Dockyu, et al. 2011. Anthranilate degradation by an eurypsychrophilic Pseudomonas sp. form Alps. 한국미생물학회연합회. 한국미생물학회연합회. 2011.10.14~.
The new alpine soil bacterium Pseudomonas sp. strain KOPRI 25931 was selected for the excellent ability to grow on anthranilate (2-aminobenzoate) and benzoate from Polar and Alpine Microbial Collection (PAMC) in Korea Polar Research Institute (KOPRI). It was characterized to be eurypsychrophilic (both psychrophilic and mesotolerant) with a broad temperature range of 5  30oC for benzoate degradation and cell growth. Except anthranilate and benzoate, KOPRI 25931 was capable of growth on only catechol among mono- substituted benzoates and central metabolites tested. Crude lysate from anthranilate- grown KOPRI 25931 cells showed catechol 1,2-dioxygenase activity against catechol. The genes encoding the initial steps in anthranilate metabolism were cloned in a fosmid clone, sequenced, and analyzed to reveal the six catabolic and two regulatory genes (catACBR and antRABC), which are tightly clustered within an approximately 7.8 kb-region on chromosome. When expressed in Escherichia coli, AntABC (anthranilate 1,2- dioxygenase) transformed anthranilate into catechol, and CatA (catechol 1,2-dioxygenase) showed higher ortho-cleavage activity against catechol than others. The growth test, metabolite identification, and enzyme assay demonstrate that AntABC in KOPRI 25931 oxidizes anthranilate to catechol, which is further degraded to cis,cis-muconate through ortho-cleavage pathway initi
Conference Name
Conference Place
Conference Date
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.