Screening and characterization for cold-active protease-producing bacteria from Bikal Lake
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- Screening and characterization for cold-active protease-producing bacteria from Bikal Lake
- Other Titles
- Bikal 호수로부터 저온활성 단백질분해효소 생산균주의 스크리닝 및 특성연구
- Kang, Pilsung
Lee, Yung Mi
Park, Ha Ju
Yim, Joung Han
- Bikal; bacteria; cold-active; protease; screening
- Issue Date
- Kang, Pilsung, et al. 2011. Screening and characterization for cold-active protease-producing bacteria from Bikal Lake. 한국미생물학회. 한국미생물학회. 2011.05.12~.
- The Korea Polar Research Institute (KOPRI) had collected heterotrophic bacterial strains
from water and ice of Baikal Lake. To identify excellent protease-producers among the
bacterial collection (50 strains), 43 strains were examined for their extracellular
protease activity on R2A plates supplemented with 1% skim milk at various temperatures
(4-20 C). This rapid and direct screening method permitted the selection of a small
group of 3 cold-adapted bacterial strains that showed proteolytic activities even at low
temperature of 4oC. The cold-active proteases from these strains exhibited relative
activities of 32%, 19%, and 15% at 5 C, respectively, compared to 100% activity at an
optimal temperature of 40oC, and the molecular weights of the proteases were all
estimated to be 75,000 Da by zymography. The proteases were classified into four
categories (serine protease, aspartic protease, cysteine protease, and metalloprotease)
according to the extent of enzymatic inhibition by a class-specific protease inhibitor.
Since highly active and/or cold-adapted proteases have a potential for industrial or
commercial enzymes, the protease-producing bacteria selected in this work will be
studied as a valuable natural source for new proteases. This work was supported by a
grant to KOPRI (PE11060).
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