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Microbial diversity of active layer soil from the Canadian high Arctic

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Microbial diversity of active layer soil from the Canadian high Arctic
Other Titles
캐나다 북극 활동층의 미생물 다양성
Kim, Ok-Sun
Lee, Yoo Kyung
Kim, Hye Min
Lee, Bang Yong
Chun, Jongsik
Hong, Soon Gyu
Active layer; Bacteroidetes; Euryarchaeota; Proteobacteria; Thaumarchaeota
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Kim, Ok-Sun, et al. 2011. Microbial diversity of active layer soil from the Canadian high Arctic. LPSC. LPSC. 2011.03.10~.
Active layer is the surface layer of permafrost, which is an extraterrestrial analogue. The repeats of thawing and freezing make active layer an extreme environment for microorganisms. The bacterial and archaeal diversity in an active layer soil from the Canadian high Arctic was analyzed by using a high resolution pyrosequencing analysis. Since last few years, DNA sequencing-based microbial community studies have been widely propagated by the invention of pyrosequencing which produce millions of sequences in a single run (Ronaghi, 2001). This can exclude the time-consuming step such as constructing clone libraries and generates millions of sequencing in a single run. Therefore, tremendous sequence information in a sample can make be visualized the deep insights of microbial population. Total 7,796 bacterial reads for 40 phyla and 245 archaeal reads for 4 phyla were collected, reflecting the high diversity of bacteria. Much higher diversity in bacteria than in archaea was confirmed from the statistical analysis. Among these 40 phyla, 22 phyla were assigned as well-known phyla such as Proteobacteria and Bacteroidetes, whereas 18 phyla assigned as candidate phyla such as TM7, OD1. Proteobacteria (most were from the class Alphaproteobactera and Betaproteobacteria) and Bacteroidetes encompassed the majority of sequences with 67.8%. This is shown a good agreement of soil microbial communities in four different types of soils from Brazil, Florida, Illinois and Canada (Roesch et al., 2007), despite of targeting different region of 16S rRNA gene with this study. A good agreement was found that phylum Proteobacteria was the most dominant with 27.6 – 58.2% in all environments. Bacteroidetes was detected with 30.0% in case of Canadian permafrost, however, around 10% in other environments. The majority of the sequences from rhizosphere soil on Antarctica were affiliated to the Actinobacteria (28.7%) that were typically dominated in soil environment. In case of Archaea, rel
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