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BACTERIAL COMMUNITY OF PERMAFROST SOIL IN ALASKA BASED ON 16S RRNA GENE BY PYROSEQUENCING METHOD

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Title
BACTERIAL COMMUNITY OF PERMAFROST SOIL IN ALASKA BASED ON 16S RRNA GENE BY PYROSEQUENCING METHOD
Other Titles
알래스카 영구동토층 토양의 세균 군집구조
Authors
Nam, Sungjin
Lee, Bang Yong
Han, Duk Ki
Kim, Hye Min
Kim, Ok-Sun
Lee, Yoo Kyung
Chun, Jongsik
Keywords
microbial diversitypermafrostpyrosequencing
Issue Date
2011
Citation
Nam, Sungjin, et al. 2011. BACTERIAL COMMUNITY OF PERMAFROST SOIL IN ALASKA BASED ON 16S RRNA GENE BY PYROSEQUENCING METHOD. IASC, KOPRI ect.. IASC, KOPRI ect.. 2011.03.28~.
Abstract
Despite the potential contribution of thawing permafrost soil to the global climate change, the research of the microbial diversity in this habitat has been scarce. In this study, we investigate the bacterial diversity in active layer soil from the Alaskan high Arctic based on 16S rRNA genes using pyrosequencing. Although the bacterial diversity in this habitat was relatively low compared to other temperate soil environments, 16 known divisions and 8 unknown divisions were detected, where the phyla of Proteobacteria (32.0%), Acidobacteria (24.5%) and Actinobacteria (20.9%) were dominant. The most frequent sequences were assigned as a EU150278_s belonged to the order Xanthomoadales of the class Gammaproteobacteria with 3.4% in species level. These bacterial habitats contained approximately 1,384 phylotypes. Species richness showed 2,021 by Chao and 2,180 by ACE, whereas species evenness presented 6.18 and 0.004 by Shannon and Simpson, respectively. We also reveal the presence of bacterial families with the members including environmental relatives retrieved from diverse environments such as different types of soil habitats, drinking water biofilm, seawater, freshwater habitats and the atmosphere. Finally, we expect that complex environmental factors could affect shaping the microbiological composition of this environment. In conclusion, bacterial community in this ecosystem can be readily monitored by pyrosequencing of 16S rRNA gene.
URI
https://repository.kopri.re.kr/handle/201206/8518
Conference Name
IASC, KOPRI ect.
Conference Place
IASC, KOPRI ect.
Conference Date
2011.03.28~
Type
Proceeding
Indexed
Pro(초록)국외
Appears in Collections  
2011-2011, Korea Arctic Multidisciplinary Program(KAMP): Research on the Arctic Climate & Environment Change (11-11) / Yoon, Young Jun (PP11020)
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