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Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components

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Title
Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components
Other Titles
배지 성분의 통계적 최적화를 이용한 북극 미세조류 Chlamydomonas sp. KNM0029C의 지질 생산 증대
Authors
Kim, Eun Jae
Jung, Woongsic
Kim, Sanghee
Choi, Han-Gu
Han, Se Jong
Subject
Ergonomics
Keywords
Arctic microalgae; Chlamydomonas sp. KNM0029C; Lipid; Response surface methodology; Statistical optimization; Tris-acetate-phosphate
Issue Date
2019-09
Citation
Kim, Eun Jae, et al. 2019. "Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components". KSBB Journal, 34(3): 173-177.
Abstract
A sequential statistical optimization was applied tothe components of Tris-acetate-phosphate (TAP) medium, toenhance the cell density and lipid production of Arcticmicroalga Chlamydomonas sp. KNM0029C. The evaluationwas preformed through an elimination process and Plackett-Burman design to select for significant factors, contributingtoward cell growth. NH4Cl, MgSO4·7H2O, potassium phosphate,and trace elements were established as the effectivecomponents and used as variable factors in Box-Behnken designtest, - a response surface methodology (RSM). The final optimizedconcentrations were 0.54, 0.16, 0.04, and 0.08 g/L ofNH4Cl, MgSO4·7H2O, potassium phosphate, and trace elements,respectively. Overall, 9.9% enhanced lipid productionwas achieved by using optimized TAP medium at 4°C. Theresults of the present study could potentially contributetoward large-scale lipid production at low temperatures.
URI
https://repository.kopri.re.kr/handle/201206/10502
DOI
http://dx.doi.org/10.7841/ksbbj.2019.34.3.173
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