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Crystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14

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Title
Crystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14
Other Titles
스포어를 형성하는 빙하미생물 (Paenisporosarcina sp. TG-14) 유래 PaGerE 단백질의 구조연구
Authors
Lee, Chang Woo
Park, Sun-Ha
Koh, Hye Yeon
Jeong, Chang Sook
Hwang, Jisub
Lee, Sung Gu
Youn, Ui Joung
Lee, Chang Sup
Park, Hyun Ho
Kim, Hak Jun
Park, Hyun
Lee, Jun Hyuck
Subject
Biochemistry & Molecular Biology; Biophysics
Keywords
GerE; Paenisporosarcina sp. TG-14; X-ray crystallography; spore
Issue Date
2019-05
Citation
Lee, Chang Woo, et al. 2019. "Crystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14". BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 513(2): 374-379.
Abstract
In cold and harsh environments such as glaciers and sediments in ice cores, microbes can survive by forming spores. Spores are composed of a thick coat protein, which protects against external factors such as heat-shock, high salinity, and nutrient deficiency. GerE is a key transcription factor involved in spore coat protein expression in the mother cell during sporulation. GerE regulates transcription during the late sporulation stage by directly binding to the promoter of cotB gene. Here, we report the crystal structure of PaGerE at 2.09 A resolution from Paenisporosarcina sp. TG-14, which was isolated from the Taylor glacier. The PaGerE structure is composed of four α-helices and adopts a helix-turn-helix architecture with 68 amino acid residues. Based on our DNA binding analysis, the PaGerE binds to the promoter region of CotB to affect protein expression. Additionally, our structural comparison studies suggest that DNA binding by PaGerE causes a conformational change in the α4-helix region, which may strongly induce dimerization of PaGerE.
URI
https://repository.kopri.re.kr/handle/201206/10895
DOI
http://dx.doi.org/10.1016/j.bbrc.2019.04.019
Appears in Collections  
2019-2019, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (19-19) / Kim, Jin-Hyoung (PE19080)
2019-2019, Development of potential candidates as antibiotics based on polar genetic resources (19-19) / Lee, Jun Hyuck (PE19210)
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