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Effect of growth rate on transcriptomic responses to immune stimulation in wild-type, domesticated, and GH-transgenic coho salmon

Cited 1 time in wos
Cited 2 time in scopus
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Title
Effect of growth rate on transcriptomic responses to immune stimulation in wild-type, domesticated, and GH-transgenic coho salmon
Other Titles
야생, 육종 및 유전자변형 연어의 성장률에 따른 전사체에 미치는 면역반응의 차이
Authors
Kim, Jin-Hyoung
Macqueen, Daniel J.
Winton, James R.
Hansen, John D.
Park, Hyun
Devlin, Robert H.
Subject
Biotechnology & Applied Microbiology; Genetics & Heredity
Keywords
Growth; PGN; Poly I; coho salmon; growth hormone; immunity; pleiotropy; selective breeding; transcriptomics; transgenesis; c
Issue Date
2019-12
Citation
Kim, Jin-Hyoung, et al. 2019. "Effect of growth rate on transcriptomic responses to immune stimulation in wild-type, domesticated, and GH-transgenic coho salmon". BMC GENOMICS, 20(1024): 1-16.
Abstract
RNA-Seq was performed on liver and head kidney in four 'growth response groups'injected with polyinosinic-polycytidylic acid (Poly I:C; viral mimic), peptidoglycan (PGN;bacterial mimic) or PBS (control). These groups were: 1) 'W': wild-type, 2) 'TF': growth hormone (GH) transgenic salmon with ~3-fold higher growth-rate than W, 3) 'TR': GH transgenic fish ration restricted to possess a growth-rate equal to W, and 4) 'D': domesticated non-transgenic fish showing growth-rate intermediate to W and TF. D and TF showed a higher similarity in transcriptomic response compared to W and TR. Several immune genes showed constitutive expression differences among growth response groups, including perforin 1 and C-C motif chemokine 19-like. Among the affected immune pathways, most were up-regulated by Poly I:C and PGN. In response to PGN, the c-type lectin receptor signalling pathway responded uniquely in TF and TR. In response to stimulation with both immune mimics, TR responded more strongly than other groups. Further, group-specific pathway responses to PGN stimulation included NOD-like receptor signalling in W and platelet activation in TR. TF consistently showed the most attenuated immune response relative to W, and more DEGs were apparent in TR than TF and D relative to W, suggesting that a non-satiating ration coupled with elevated circulating GH levels may cause TR to possess enhanced immune capabilities. Alternatively, TF and D salmon are prevented from acquiring the same level of immune response as TR due to direction of energy to high overall somatic growth.
URI
https://repository.kopri.re.kr/handle/201206/10985
DOI
http://dx.doi.org/10.1186/s12864-019-6408-4
Appears in Collections  
2019-2019, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (19-19) / Kim, Jin-Hyoung (PE19080)
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