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Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology

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Title
Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology
Other Titles
3세대 염기서열 및 Hi-C 분석기법으로 남극이빨고기의 염색체 유전자 해독
Authors
Lee, Seung Jae
Kim, Jeong-Hoon
Jo, Euna
Choi, Eunkyung
Kim, Jinmu
Choi, Seok-Gwan
Chung, Sangdeok
Kim, Hyun-Woo
Park, Hyun
Subject
Zoology
Keywords
Antarctic toothfish; Dissostichus mawsoni; Genome assembly; Hi-C; Pacific Biosciences
Issue Date
2021-01
Citation
Seung Jae Lee, et al. 2021. "Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology". ZOOLOGICAL RESEARCH, 42(1): 124-129.
Abstract
The Antarctic toothfish, Dissostichus mawsoni, belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of D. mawsoni, which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of D. mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species
URI
https://repository.kopri.re.kr/handle/201206/11772
DOI
http://dx.doi.org/10.24272/j.issn.2095-8137.2020.264
Appears in Collections  
2019-2020, Ecosystem Structure and Function of Marine Protected Area (MPA) in Antarctica (19-20) / Kim, Jeong-Hoon (PE20090)
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