Effects of Nitrogen Limitation on Phytoplankton Physiology in the Western Arctic Ocean in Summer

Abstract

Phytoplankton in the Arctic Ocean are subject to nitrogen limitation in the summer, however, how severely the nitrogen stress affects phytoplankton physiology remains largely unknown. In the summers of 2015-2018, we examined the distribution of phytoplankton photophysiological properties across two contrasting regions of the Arctic Ocean with distinctly different levels of nitrogen availability in the upper water column. We quantified the extent of nitrogen stress using a highly sensitive fluorescence induction and relaxation system to obtain continuous underway measurements and via discrete sample analyses of phytoplankton physiology, as well as nutrient enrichment incubations. The results revealed vast regions in the Chukchi Sea where phytoplankton photosynthesis was severely nitrogen-stressed. Thereby, the maximum quantum yield of photochemistry in photosystem II showed only a small decrease (12 +/- 9%) relative to its nutrient-replete values, while the maximum photosynthetic electron transport rates under saturating irradiance were impaired to a greater extent (40 +/- 17%). This phytoplankton photosynthesis response is indicative of a severe nitrogen limitation, which results in dramatic reduction in growth and net primary production rates. Nutrient enrichment incubations also revealed a marked increase in large-size phytoplankton growth (>10 mu m) after the nitrogen stress was alleviated, suggesting that the larger cells were more susceptible to nitrogen stress. These results are important for understanding how regional nitrogen fluxes control variability in the primary production and phytoplankton community structure and how these processes might change with rapid climate changes in the Arctic Ocean.