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Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

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Title
Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model
Other Titles
남극 지의류 Umbilicaria Antarctica 추출물이 RAW264.7 세포와 zebrafish 모델에서의 항염증 효능
Authors
Hong, Ju-Mi
Kim, Jung Eun
Min, Seul Ki
Kim, Kyung Hee
Han, Se Jong
Yim, Joung Han
Park, Hyun
Kim, Jin-Hyoung
Kim, Il-Chan
Subject
Biotechnology & Applied Microbiology; Research & Experimental Medicine
Keywords
Antarctic lichen; Anti-inflammation; Umbilicaria Antarctica
Issue Date
2021-02
Citation
Hong, Ju-Mi, et al. 2021. "Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model". BIOMED RESEARCH INTERNATIONAL, 2021(1): 1-12.
Abstract
Umbilicaria antarctica (UA) is a member of the family Umbilicariaceae. To the best of our knowledge, no studies on its anti-inflammatory effects have been reported yet. In the present study, we examined its ability to suppress inflammatory responses and the molecular mechanisms underlying these abilities using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model of inflammation. We investigated the effects of UA on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW 264.7 cells. To explore the anti-inflammatory mechanisms of UA, we measured the mRNA and protein expression of pro-inflammatory mediators in LPS-stimulated RAW 264.7 cells using quantitative RT-PCR and western blot analyses, respectively. UA significantly inhibited the production of NO, PGE2, interleukin (IL)-6, and tumor necrosis factor (TNF)-α in the LPS-stimulated RAW 264.7 cells. It also suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor (NF)-κB activation in LPS-stimulated RAW 264.7 cells and tail pin-cutting induced zebrafish model. Collectively, these findings indicate that UA significantly inhibits LPS-stimulated inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. Overall, our results demonstrate that UA extract exerts strong anti-inflammatory activities in in vitro and in vivo models and suggest that UA may be an effective novel therapeutic agent for the treatment of inflammatory diseases.
URI
https://repository.kopri.re.kr/handle/201206/11953
DOI
http://dx.doi.org/10.1155/2021/8812090
Appears in Collections  
2021-2021, Commercialization of new Biomaterials from polar organisms (21-21) / Yim, Joung Han (PE21150)
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