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Enzymatic synthesis of anabolic steroids glycosides by glucosyltransferase from Terribacillus sp. PAMC 23288

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Title
Enzymatic synthesis of anabolic steroids glycosides by glucosyltransferase from Terribacillus sp. PAMC 23288
Other Titles
북극 토양 미생물 (Terribacillus sp. PAMC 23288) 유래 glucosyltransferase 효소를 이용한 스테로이드 기질의 효소적 당화 반응 연구
Authors
Yu, Eun-Ji
Yamaguchi, Tokutaro
Lee, Joo-Ho
Lim, A-Rang
Lee, Jun Hyuck
Park, Hyun
Oh, Tae-Jin
Subject
Biotechnology & Applied Microbiology; Microbiology
Keywords
Testosterone; nandrolone; glucosylation; neuroprotective activity; rotenone-induced apoptosis; steroid
Issue Date
2020-04
Citation
Yu, Eun-Ji, et al. 2020. "Enzymatic synthesis of anabolic steroids glycosides by glucosyltransferase from Terribacillus sp. PAMC 23288". JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 30(4): 604-614.
Abstract
To date, the use of steroid drugs has been steadily thanks to its effects, but due to severe side effects, studies on the derivative activity of steroids are being conducted. Nandrolone, a breast cancer drug, cortisone and prednisone which reduce inflammation, pain, and edema used in this study are not known as sugar derivatives. O-glucose was successfully linked using UDP-glucosyltransferase (UGT-1), and bioactivity was tested in vitro. NMR analysis found that those products produced by the nandrolone and testosterone were 17β-nandrolone β-D-glucose and 17β-testosterone β-D-glucose, respectively. In addition, testosterone derivatives in rotenone-treated PC12 cells showed higher cell viability and no cytotoxicity compared to control. In the presence of H2O2 in SH-SY5Y cells, nandrolone derivatives showed cell viability and attenuated cytotoxicity. Both derivatives also increased the renal protective effect and cellular ATP.
URI
https://repository.kopri.re.kr/handle/201206/12092
DOI
http://dx.doi.org/10.4014/jmb.1911.11057
Appears in Collections  
2019-2019, Development of potential candidates as antibiotics based on polar genetic resources (19-19) / Lee, Jun Hyuck (PE19210)
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