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Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation

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Title
Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation
Other Titles
과산화수소 저항성을 가지는 CYP105D18 효소의 구조 및 기능 연구: 파파베린 약물의 N-oxidation 변형에 사용
Authors
Pardhe, Bashu Dev
Do, Hackwon
Jeong, Chang-Sook
Kim, Ki-Hwa
Lee, Jun Hyuck
Oh, Tae-Jin
Subject
ChemistryCrystallographyMaterials Science
Keywords
CYP105D18H2O2 toleranceStreptomyces laurentiipapaverine N-oxideenzyme mechanismscrystal morphologyco-crystals
Issue Date
2021-07
Citation
Bashu Dev Pardhe, et al. 2021. "Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation". IUCRJ, 8(4): 684-694.
Abstract
The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study was conducted to investigate the newly identified H2O2-mediated CYP105D18 from Streptomyces laurentii as the first bacterial CYP for N-oxidation. The catalytic efficiency of CYP105D18 for papaverine N-oxidation was 1.43 sec-1 μM-1. The heme oxidation rate (k) was low (<0.3 min-1) in the presence of 200 mM H2O2. This high H2O2 tolerance capacity of CYP105D18 led to higher turnover prior to heme oxidation. Additionally, the high-resolution papaverine complexed structure and substrate-free structure of CYP105D18 were determined. Structural analysis and activity assay results revealed that CYP105D18 had a strong substrate preference for papaverine because of its bendable structure. These findings establish a basis for biotechnological applications of CYP105D18 in the pharmaceutical and medicinal industries.
URI
https://repository.kopri.re.kr/handle/201206/12977
DOI
http://dx.doi.org/10.1107/S2052252521005522
Type
Article
Station
해당사항없음
Indexed
SCIE
Appears in Collections  
2021-2021, Development of potential candidates as antibiotics based on polar genetic resources (21-21) / Lee, Jun Hyuck (PM21030)
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